Studying transcription initiation by RNA polymerase with diffusion‐based single‐molecule fluorescence
Sign Up to like & getrecommendations! Published in 2017 at "Protein Science"
DOI: 10.1002/pro.3160
Abstract: Over the past decade, fluorescence‐based single‐molecule studies significantly contributed to characterizing the mechanism of RNA polymerase at different steps in transcription, especially in transcription initiation. Transcription by bacterial DNA‐dependent RNA polymerase is a multistep process… read more here.
Keywords: transcription; transcription initiation; single molecule; rna polymerase ... See more keywords
GNE-371, a Potent and Selective Chemical Probe for the Second Bromodomains of Human Transcription-Initiation-Factor TFIID Subunit 1 and Transcription-Initiation-Factor TFIID Subunit 1-like.
Sign Up to like & getrecommendations! Published in 2018 at "Journal of medicinal chemistry"
DOI: 10.1021/acs.jmedchem.8b01225
Abstract: The biological functions of the dual bromodomains of human transcription-initiation-factor TFIID subunit 1 (TAF1(1,2)) remain unknown, although TAF1 has been identified as a potential target for oncology research. Here, we describe the discovery of a… read more here.
Keywords: factor tfiid; transcription initiation; initiation factor; tfiid subunit ... See more keywords
Real-time observation of polymerase-promoter contact remodeling during transcription initiation
Sign Up to like & getrecommendations! Published in 2017 at "Nature Communications"
DOI: 10.1038/s41467-017-01041-1
Abstract: Critical contacts made between the RNA polymerase (RNAP) holoenzyme and promoter DNA modulate not only the strength of promoter binding, but also the frequency and timing of promoter escape during transcription. Here, we describe a… read more here.
Keywords: transcription; real time; polymerase; promoter ... See more keywords
The route to transcription initiation determines the mode of transcriptional bursting in E. coli
Sign Up to like & getrecommendations! Published in 2020 at "Nature Communications"
DOI: 10.1038/s41467-020-16367-6
Abstract: Transcription is fundamentally noisy, leading to significant heterogeneity across bacterial populations. Noise is often attributed to burstiness, but the underlying mechanisms and their dependence on the mode of promotor regulation remain unclear. Here, we measure… read more here.
Keywords: transcription; route transcription; transcription initiation; regulated via ... See more keywords
The dynamic landscape of transcription initiation in yeast mitochondria
Sign Up to like & getrecommendations! Published in 2020 at "Nature Communications"
DOI: 10.1038/s41467-020-17793-2
Abstract: Controlling efficiency and fidelity in the early stage of mitochondrial DNA transcription is crucial for regulating cellular energy metabolism. Conformational transitions of the transcription initiation complex must be central for such control, but how the… read more here.
Keywords: yeast mitochondria; transcription; initiation; dynamic landscape ... See more keywords
Eukaryotic core promoters and the functional basis of transcription initiation
Sign Up to like & getrecommendations! Published in 2018 at "Nature Reviews Molecular Cell Biology"
DOI: 10.1038/s41580-018-0028-8
Abstract: RNA polymerase II (Pol II) core promoters are specialized DNA sequences at transcription start sites of protein-coding and non-coding genes that support the assembly of the transcription machinery and transcription initiation. They enable the highly… read more here.
Keywords: transcription; gene; transcription initiation; core promoters ... See more keywords
An SI3-σ arch stabilizes cyanobacteria transcription initiation complex
Sign Up to like & getrecommendations! Published in 2022 at "Proceedings of the National Academy of Sciences of the United States of America"
DOI: 10.1073/pnas.2219290120
Abstract: Multi-subunit RNA polymerases (RNAPs) associate with initiation factors (σ in bacteria) to start transcription. The σ factors are responsible for recognizing and unwinding promoter DNA in all bacterial RNAPs. Here, we report two cryo-EM structures… read more here.
Keywords: transcription; transcription initiation; si3 arch; initiation ... See more keywords
Impaired function of rDNA transcription initiation machinery leads to derepression of ribosomal genes with insertions of R2 retrotransposon
Sign Up to like & getrecommendations! Published in 2022 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkab1276
Abstract: Abstract Eukaryotic genomes harbor hundreds of rRNA genes, many of which are transcriptionally silent. However, little is known about selective regulation of individual rDNA units. In Drosophila melanogaster, some rDNA repeats contain insertions of the… read more here.
Keywords: insertions retrotransposon; derepression; rdna transcription; rdna units ... See more keywords
Conformational heterogeneity and bubble dynamics in single bacterial transcription initiation complexes
Sign Up to like & getrecommendations! Published in 2018 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkx1146
Abstract: Abstract Transcription initiation is a major step in gene regulation for all organisms. In bacteria, the promoter DNA is first recognized by RNA polymerase (RNAP) to yield an initial closed complex. This complex subsequently undergoes… read more here.
Keywords: transcription; dna; bubble dynamics; conformational heterogeneity ... See more keywords
Genome-wide mapping reveals conserved and diverged R-loop activities in the unusual genetic landscape of the African trypanosome genome
Sign Up to like & getrecommendations! Published in 2018 at "Nucleic Acids Research"
DOI: 10.1093/nar/gky928
Abstract: Abstract R-loops are stable RNA–DNA hybrids that have been implicated in transcription initiation and termination, as well as in telomere maintenance, chromatin formation, and genome replication and instability. RNA Polymerase (Pol) II transcription in the… read more here.
Keywords: genome wide; transcription; conserved diverged; genome ... See more keywords
Pervasive and dynamic transcription initiation in Saccharomyces cerevisiae.
Sign Up to like & getrecommendations! Published in 2019 at "Genome research"
DOI: 10.1101/gr.245456.118
Abstract: Transcription initiation is finely regulated to ensure proper expression and function of genes. The regulated transcription initiation in response to various environmental stimuli in a classic model organism Saccharomyces cerevisiae has not been systematically investigated.… read more here.
Keywords: transcription; saccharomyces cerevisiae; transcription initiation; core promoters ... See more keywords