Articles with "transcription polymerase" as a keyword



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MET 14 Skipping Mutation in Pulmonary Sarcomatoid Carcinoma Using Reverse Transcription Polymerase Chain Reaction Method: P117

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Published in 2018 at "Journal of Thoracic Oncology"

DOI: 10.1016/j.jtho.2018.10.143

Abstract: Background: Pulmonary sarcomatoid carcinoma (PSC) is a recognized category of highly aggressive and poorly differentiated non-small-cell lung carcinoma (NSCLC), with five different subtypes: pleomorphic, spindle, giant cell, carcinosarcoma, and pulmonary blastoma. Although uncommon (0.1% to… read more here.

Keywords: carcinoma; met skipping; pulmonary sarcomatoid; reverse transcription ... See more keywords
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Amplification of cDNA Generated by Reverse Transcription of mRNA: Two-Step Reverse Transcription-Polymerase Chain Reaction (RT-PCR).

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Published in 2019 at "Cold Spring Harbor protocols"

DOI: 10.1101/pdb.prot095190

Abstract: Reverse transcription-polymerase chain reaction (RT-PCR) is a powerful method to detect and synthesize cDNA copies of low-copy-number mRNAs. Two enzymes are used: reverse transcriptase to produce single-stranded cDNA copies, which are then used as templates… read more here.

Keywords: transcription; pcr; reaction; reverse transcription ... See more keywords
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Fabrication of an Oscillating Thermocycler to Analyze the Canine Distemper Virus by Utilizing Reverse Transcription Polymerase Chain Reaction

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Published in 2022 at "Micromachines"

DOI: 10.3390/mi13040600

Abstract: The reverse transcription-polymerase chain reaction (RT-PCR) has been utilized as an effective tool to diagnose the infectious diseases of viruses. In the present work, the oscillating thermocycler is fabricated and performed to carry out the… read more here.

Keywords: oscillating thermocycler; reaction; thermocycler; transcription polymerase ... See more keywords
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Detection of Feline Coronavirus in Feline Effusions by Immunofluorescence Staining and Reverse Transcription Polymerase Chain Reaction

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Published in 2020 at "Pathogens"

DOI: 10.3390/pathogens9090698

Abstract: Feline coronavirus (FCoV), the pathogen for feline infectious peritonitis, is a lethal infectious agent that can cause effusions in the pleural and abdominal cavities in domestic cats. To study the epidemiology of FCoV in Taiwan,… read more here.

Keywords: sick cats; staining reverse; feline coronavirus; reverse transcription ... See more keywords
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Application and Comparative Evaluation of Fluorescent Antibody, Immunohistochemistry and Reverse Transcription Polymerase Chain Reaction Tests for the Detection of Rabies Virus Antigen or Nucleic Acid in Brain Samples of Animals Suspected of Rabies in India

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Published in 2018 at "Veterinary Sciences"

DOI: 10.3390/vetsci5010024

Abstract: Accurate and early diagnosis of animal rabies is critical for undertaking public health measures. Whereas the direct fluorescent antibody (DFA) technique is the recommended test, the more convenient, direct rapid immunochemistry test (dRIT), as well… read more here.

Keywords: brain samples; chain reaction; reverse transcription; polymerase chain ... See more keywords
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SARS-CoV-2 Reverse Transcription-Polymerase Chain Reaction Positivity and Seroprevalence among Health Care Workers in a Referral Cancer Institute: A Cross-sectional Study

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Published in 2022 at "Open Access Macedonian Journal of Medical Sciences"

DOI: 10.3889/oamjms.2022.8066

Abstract: BACKGROUND: During the ongoing coronavirus disease 2019 pandemic, healthcare workers (HCWs) are presumed to be at increased risk of infection by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), transmitting the infection to vulnerable patients if… read more here.

Keywords: sars cov; referral cancer; positivity; transcription polymerase ... See more keywords
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Comparative study between an antigen test and the reverse transcription-polymerase chain reaction (RT-PCR) test for the diagnosis of COVID-19 in pediatrics.

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Published in 2023 at "Archivos argentinos de pediatria"

DOI: 10.5546/aap.2022-02908.eng

Abstract: Introduction. The COVID-19 pandemic has brought to light the need for rapid diagnostic tests. The gold standard test is reverse transcription-polymerase chain reaction (RT-PCR). RT-PCR requires equipment and trained personnel, and results may take a… read more here.

Keywords: antigen; reverse transcription; test reverse; transcription polymerase ... See more keywords