Implication of Pseudo Reference Genes in Normalization of Data from Reverse Transcription-Quantitative PCR.
Sign Up to like & getrecommendations! Published in 2020 at "Gene"
DOI: 10.1016/j.gene.2020.144948
Abstract: Pseudogenes are duplicated or retrotransposed DNA sequences of native functional genes. Amplification of pseudogenes along with gene of interest often produces false positive results, which is an innate problem in Reverse Transcription-quantitative PCR (RT-qPCR). Selecting… read more here.
Keywords: quantitative pcr; transcription quantitative; reference; reverse transcription ... See more keywords
Screening potential reference genes for reverse transcriptionâquantitative polymerase chain reaction normalization in Atrijuglans hetaohei (Lepidoptera: Gelechioidea)
Sign Up to like & getrecommendations! Published in 2020 at "Entomological Research"
DOI: 10.1111/1748-5967.12444
Abstract: Atrijuglans hetaohei Yang (Lepidoptera: Gelechioidea), is one of the major pests that seriously damage the walnut fruits. Although the morphology and physiology of A. hetaohei have been widely studied, suitable reference genes for normalizing target… read more here.
Keywords: lepidoptera gelechioidea; transcription quantitative; reference; reverse transcription ... See more keywords
Development and validation of a reverse transcription quantitative polymerase chain reaction for tilapia lake virus detection in clinical samples and experimentally challenged fish.
Sign Up to like & getrecommendations! Published in 2018 at "Journal of fish diseases"
DOI: 10.1111/jfd.12708
Abstract: Tilapia lake virus (TiLV) is an emerging pathogen associated with high mortalities of wild and farm-raised tilapia in different countries. In this study, a SYBR green-based reverse transcription quantitative polymerase chain reaction (RT-qPCR) assay targeting… read more here.
Keywords: detection; transcription quantitative; tilv; reverse transcription ... See more keywords
Pan-Piscine Orthoreovirus (PRV) Detection Using Reverse Transcription Quantitative PCR
Sign Up to like & getrecommendations! Published in 2021 at "Pathogens"
DOI: 10.3390/pathogens10121548
Abstract: Piscine orthoreovirus (PRV) infects farmed and wild salmon and trout species in North America, South America, Europe, and East Asia. PRV groups into three distinct genotypes (PRV-1, PRV-2, and PRV-3) that can vary in distribution,… read more here.
Keywords: orthoreovirus prv; piscine orthoreovirus; detection; transcription quantitative ... See more keywords