Articles with "transcription recombinase" as a keyword



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Use of a rapid reverse-transcription recombinase aided amplification assay for respiratory syncytial virus detection.

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Published in 2018 at "Diagnostic microbiology and infectious disease"

DOI: 10.1016/j.diagmicrobio.2017.10.005

Abstract: In this study, a rapid reverse-transcription recombinase aided amplification (RT-RAA) assay was developed to detect respiratory syncytial virus (RSV) subgroups A and B, respectively. The reaction was performed at 39°C in less than 30min. The… read more here.

Keywords: rapid reverse; recombinase aided; transcription recombinase; aided amplification ... See more keywords
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Rapid detection of peach latent mosaic viroid by reverse transcription recombinase polymerase amplification.

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Published in 2020 at "Molecular and cellular probes"

DOI: 10.1016/j.mcp.2020.101627

Abstract: Reverse transcription recombinase polymerase amplification (RT-RPA), an isothermal nucleic acid amplification and detection method, was developed to detect peach latent mosaic viroid (PLMVd) in pollen and peach leaves. Results showed that this RT-RPA detection method… read more here.

Keywords: detection; transcription recombinase; peach; recombinase polymerase ... See more keywords
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Point-of-Care Testing for Norovirus Typing Using CRISPR/Cas12a Combined with Reverse Transcription Recombinase Polymerase Amplification.

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Published in 2023 at "Bioconjugate chemistry"

DOI: 10.1021/acs.bioconjchem.3c00181

Abstract: Noroviruses (NoVs) are one of the leading causes of acute gastroenteritis in humans. This study combined reverse transcription recombinase polymerase amplification (RT-RPA) with a clustered regularly interspaced short palindromic repeat/CRISPR-associated protein (CRISPR/Cas) nucleic acid detection… read more here.

Keywords: transcription recombinase; detection; combined reverse; polymerase amplification ... See more keywords
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Rapid detection of SARS-CoV-2 by low volume real-time single tube reverse transcription recombinase polymerase amplification using an exo probe with an internally linked quencher (exo-IQ)

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Published in 2020 at "Clinical Chemistry"

DOI: 10.1093/clinchem/hvaa116

Abstract: Abstract Background The current outbreak of SARS-CoV-2 has spread to almost every country with more than three million confirmed cases and over two hundred thousand deaths as of April 28, 2020. Rapid first-line testing protocols… read more here.

Keywords: probe; transcription recombinase; recombinase polymerase; reverse transcription ... See more keywords
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Magnetic Field-Enhanced Agglutination Readout Combined With Isothermal Reverse Transcription Recombinase Polymerase Amplification for Rapid and Sensitive Molecular Detection of Dengue Virus

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Published in 2021 at "Frontiers in Chemistry"

DOI: 10.3389/fchem.2021.817246

Abstract: Among the numerous molecular diagnostic methods, isothermal reverse transcription recombinase polymerase amplification (RT-RPA) is a simple method that has high sensitivity and avoids the use of expensive instruments. However, detection of amplified genomes often requires… read more here.

Keywords: agglutination; transcription recombinase; recombinase polymerase; isothermal reverse ... See more keywords
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Rapid Visual Detection of Hepatitis C Virus Using Reverse Transcription Recombinase-Aided Amplification–Lateral Flow Dipstick

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Published in 2022 at "Frontiers in Cellular and Infection Microbiology"

DOI: 10.3389/fcimb.2022.816238

Abstract: Hepatitis C virus (HCV) infection is a global public health threat. Reaching the World Health Organization’s objective for eliminating viral hepatitis by 2030 will require a precise disease diagnosis. While immunoassays and qPCR play a… read more here.

Keywords: transcription recombinase; detection; hepatitis virus; virus ... See more keywords
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Establishment of a reverse transcription recombinase-aided amplification detection method for porcine group a rotavirus

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Published in 2022 at "Frontiers in Veterinary Science"

DOI: 10.3389/fvets.2022.954657

Abstract: Porcine rotavirus type A (PoRVA) is the main cause of dehydration and diarrhea in piglets, which has a great impact on the development of the pig industry worldwide. A rapid, accurate and sensitive detection method… read more here.

Keywords: recombinase aided; detection method; transcription recombinase; detection ... See more keywords