Switching ON of Transcription‐Translation System Using GUV Fusion by Co‐supplementation of Calcium with Long‐Chain Polyethylene Glycol
Sign Up to like & getrecommendations! Published in 2021 at "ChemBioChem"
DOI: 10.1002/cbic.202100100
Abstract: Giant unilamellar vesicles (GUVs) have been used as a material for bottom‐up synthetic biology. However, due to the semi‐permeability of the membrane, the need for methods to fuse GUVs has increased. To this aim, methods… read more here.
Keywords: fusion; transcription translation; guv fusion; biology ... See more keywords
A bead-based method for the removal of the amino acid lysine from cell-free transcription-translation systems.
Sign Up to like & getrecommendations! Published in 2020 at "Journal of biotechnology"
DOI: 10.1016/j.btecx.2020.100024
Abstract: Cell-free transcription-translation systems are a versatile tool to study gene expression, enzymatic reactions and biochemical regulation mechanisms. Because cell-free transcription-translation systems are often derived from cell lysates, many different substances, among them amino acids, are… read more here.
Keywords: transcription translation; free transcription; lysine; cell free ... See more keywords
Rapid and Scalable Characterization of CRISPR Technologies Using an E. coli Cell-Free Transcription-Translation System.
Sign Up to like & getrecommendations! Published in 2018 at "Molecular cell"
DOI: 10.1016/j.molcel.2017.12.007
Abstract: CRISPR-Cas systems offer versatile technologies for genome engineering, yet their implementation has been outpaced by ongoing discoveries of new Cas nucleases and anti-CRISPR proteins. Here, we present the use of E. coli cell-free transcription-translation (TXTL) systems… read more here.
Keywords: transcription translation; crispr; free transcription; cell free ... See more keywords
BDNF induces in vivo long-lasting enhancement of synaptic transmission and structural reorganization at the hippocampal mossy fibers in a transcription and translation-independent manner
Sign Up to like & getrecommendations! Published in 2020 at "Neurobiology of Learning and Memory"
DOI: 10.1016/j.nlm.2019.107125
Abstract: Brain-derived neurotrophic factor (BDNF) is an essential product of protein synthesis with a prominent impact on brain signaling and synaptic plasticity. Exogenous application of this neurotrophin is able to induce long-term potentiation (LTP) in several… read more here.
Keywords: bdnf induces; transcription translation; plasticity; structural reorganization ... See more keywords
An enhanced assay to characterize anti-CRISPR proteins using a cell-free transcription-translation system.
Sign Up to like & getrecommendations! Published in 2019 at "Methods"
DOI: 10.1016/j.ymeth.2019.05.014
Abstract: The characterization of CRISPR-Cas immune systems in bacteria was quickly followed by the discovery of anti-CRISPR proteins (Acrs) in bacteriophages. These proteins block different steps of CRISPR-based immunity and, as some inhibit Cas nucleases, can… read more here.
Keywords: transcription translation; free transcription; cell free; translation system ... See more keywords
Hybrid Nucleic Acid-Quantum Dot Assemblies as Multiplexed Reporter Platforms for Cell-Free Transcription Translation-Based Biosensors.
Sign Up to like & getrecommendations! Published in 2022 at "ACS synthetic biology"
DOI: 10.1021/acssynbio.2c00394
Abstract: Cell-free synthetic biology has emerged as a valuable tool for the development of rapid, portable biosensors that can be readily transported in the freeze-dried form to the point of need eliminating cold chain requirements. One… read more here.
Keywords: cell free; transcription translation; biology; quantum dot ... See more keywords
Tuning of Recombinant Protein Expression in Escherichia coli by Manipulating Transcription, Translation Initiation Rates, and Incorporation of Noncanonical Amino Acids.
Sign Up to like & getrecommendations! Published in 2017 at "ACS synthetic biology"
DOI: 10.1021/acssynbio.7b00019
Abstract: Protein synthesis in cells has been thoroughly investigated and characterized over the past 60 years. However, some fundamental issues remain unresolved, including the reasons for genetic code redundancy and codon bias. In this study, we… read more here.
Keywords: protein expression; transcription translation; initiation rates; translation ... See more keywords
Dynamics of transcription–translation coordination tune bacterial indole signaling
Sign Up to like & getrecommendations! Published in 2019 at "Nature Chemical Biology"
DOI: 10.1038/s41589-019-0430-3
Abstract: Indole signaling is an important cross-species communication pathway in the mammalian gut. In bacteria, upon induction by tryptophan, the molecular sensor ( tnaC ) controls indole biosynthesis by precisely coordinating dynamics of the corresponding macromolecular… read more here.
Keywords: transcription translation; transcription; indole signaling; translation coordination ... See more keywords
Ribosome reactivates transcription by physically pushing RNA polymerase out of transcription arrest
Sign Up to like & getrecommendations! Published in 2020 at "Proceedings of the National Academy of Sciences of the United States of America"
DOI: 10.1073/pnas.1919985117
Abstract: Significance Transcription and translation are coupled in bacteria, meaning that translation of the nascent mRNA by ribosome happens simultaneously with its synthesis by RNA polymerase (RNAP). Uncoupling of transcription from translation may lead to arrest… read more here.
Keywords: transcription; transcription translation; rna polymerase; translation ... See more keywords
The Untold Story of Oceanic Pilot Bartolomeu Borges who Guided Jean Ribault to Florida in 1562: Document Transcription and Translation, Accompanied by an Historical Introduction
Sign Up to like & getrecommendations! Published in 2023 at "Terrae Incognitae"
DOI: 10.1080/00822884.2023.2182986
Abstract: Until now, Portuguese pilot Bartolomeu Borges has remained an obscure figure. A new document—a lengthy letter sent by D. Alonso de Tovar, the Spanish ambassador in Portugal, to King Philip II in 1563—allows us to… read more here.
Keywords: florida 1562; bartolomeu borges; pilot bartolomeu; transcription translation ... See more keywords
AtNusG, a chloroplast nucleoid protein of bacterial origin linking chloroplast transcriptional and translational machineries, is required for proper chloroplast gene expression in Arabidopsis thaliana
Sign Up to like & getrecommendations! Published in 2022 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkac501
Abstract: Abstract In Escherichia coli, transcription-translation coupling is mediated by NusG. Although chloroplasts are descendants of endosymbiotic prokaryotes, the mechanism underlying this coupling in chloroplasts remains unclear. Here, we report transcription-translation coupling through AtNusG in chloroplasts.… read more here.
Keywords: chloroplast; transcription translation; chloroplast gene; gene expression ... See more keywords