Switching ON of Transcription‐Translation System Using GUV Fusion by Co‐supplementation of Calcium with Long‐Chain Polyethylene Glycol
Sign Up to like & getrecommendations! Published in 2021 at "ChemBioChem"
DOI: 10.1002/cbic.202100100
Abstract: Giant unilamellar vesicles (GUVs) have been used as a material for bottom‐up synthetic biology. However, due to the semi‐permeability of the membrane, the need for methods to fuse GUVs has increased. To this aim, methods… read more here.
Keywords: fusion; transcription translation; guv fusion; biology ... See more keywords
A bead-based method for the removal of the amino acid lysine from cell-free transcription-translation systems.
Sign Up to like & getrecommendations! Published in 2020 at "Journal of biotechnology"
DOI: 10.1016/j.btecx.2020.100024
Abstract: Cell-free transcription-translation systems are a versatile tool to study gene expression, enzymatic reactions and biochemical regulation mechanisms. Because cell-free transcription-translation systems are often derived from cell lysates, many different substances, among them amino acids, are… read more here.
Keywords: transcription translation; free transcription; lysine; cell free ... See more keywords
Rapid and Scalable Characterization of CRISPR Technologies Using an E. coli Cell-Free Transcription-Translation System.
Sign Up to like & getrecommendations! Published in 2018 at "Molecular cell"
DOI: 10.1016/j.molcel.2017.12.007
Abstract: CRISPR-Cas systems offer versatile technologies for genome engineering, yet their implementation has been outpaced by ongoing discoveries of new Cas nucleases and anti-CRISPR proteins. Here, we present the use of E. coli cell-free transcription-translation (TXTL) systems… read more here.
Keywords: transcription translation; crispr; free transcription; cell free ... See more keywords
BDNF induces in vivo long-lasting enhancement of synaptic transmission and structural reorganization at the hippocampal mossy fibers in a transcription and translation-independent manner
Sign Up to like & getrecommendations! Published in 2020 at "Neurobiology of Learning and Memory"
DOI: 10.1016/j.nlm.2019.107125
Abstract: Brain-derived neurotrophic factor (BDNF) is an essential product of protein synthesis with a prominent impact on brain signaling and synaptic plasticity. Exogenous application of this neurotrophin is able to induce long-term potentiation (LTP) in several… read more here.
Keywords: bdnf induces; transcription translation; plasticity; structural reorganization ... See more keywords
An enhanced assay to characterize anti-CRISPR proteins using a cell-free transcription-translation system.
Sign Up to like & getrecommendations! Published in 2019 at "Methods"
DOI: 10.1016/j.ymeth.2019.05.014
Abstract: The characterization of CRISPR-Cas immune systems in bacteria was quickly followed by the discovery of anti-CRISPR proteins (Acrs) in bacteriophages. These proteins block different steps of CRISPR-based immunity and, as some inhibit Cas nucleases, can… read more here.
Keywords: transcription translation; free transcription; cell free; translation system ... See more keywords
Peptide Coacervates Can Protect Sequestered Oligonucleotides from Nucleases and Release Them for Transcription and Translation
Sign Up to like & getrecommendations! Published in 2025 at "Biomacromolecules"
DOI: 10.1021/acs.biomac.5c00600
Abstract: We demonstrate that coacervates, membraneless organelles formed by liquid–liquid phase separation, sequester and protect short DNA reporters and a functional luciferase gene from enzymatic degradation by various nucleases. Associative coacervates, formed by electrostatic interactions between… read more here.
Keywords: protect sequestered; peptide coacervates; transcription translation; coacervates protect ... See more keywords
Leveraging Active Learning to Establish Efficient In Vitro Transcription and Translation from Bacterial Chromosomal DNA
Sign Up to like & getrecommendations! Published in 2024 at "ACS Omega"
DOI: 10.1021/acsomega.4c00111
Abstract: Gene expression is a fundamental aspect in the construction of a minimal synthetic cell, and the use of chromosomes will be crucial for the integration and regulation of complex modules. Expression from chromosomes in vitro… read more here.
Keywords: vitro transcription; transcription translation; active learning; transcription ... See more keywords
Hybrid Nucleic Acid-Quantum Dot Assemblies as Multiplexed Reporter Platforms for Cell-Free Transcription Translation-Based Biosensors.
Sign Up to like & getrecommendations! Published in 2022 at "ACS synthetic biology"
DOI: 10.1021/acssynbio.2c00394
Abstract: Cell-free synthetic biology has emerged as a valuable tool for the development of rapid, portable biosensors that can be readily transported in the freeze-dried form to the point of need eliminating cold chain requirements. One… read more here.
Keywords: cell free; transcription translation; biology; quantum dot ... See more keywords
Metabolic Perturbations to an Escherichia coli-based Cell-Free System Reveal a Trade-off between Transcription and Translation.
Sign Up to like & getrecommendations! Published in 2024 at "ACS synthetic biology"
DOI: 10.1021/acssynbio.4c00361
Abstract: Cell-free transcription-translation (TX-TL) systems have been used for diverse applications, but their performance and scope are limited by variability and poor predictability. To understand the drivers of this variability, we explored the effects of metabolic… read more here.
Keywords: trade; system; cell free; metabolic perturbations ... See more keywords
Tuning of Recombinant Protein Expression in Escherichia coli by Manipulating Transcription, Translation Initiation Rates, and Incorporation of Noncanonical Amino Acids.
Sign Up to like & getrecommendations! Published in 2017 at "ACS synthetic biology"
DOI: 10.1021/acssynbio.7b00019
Abstract: Protein synthesis in cells has been thoroughly investigated and characterized over the past 60 years. However, some fundamental issues remain unresolved, including the reasons for genetic code redundancy and codon bias. In this study, we… read more here.
Keywords: protein expression; transcription translation; initiation rates; translation ... See more keywords
A complex between IF2 and NusA suggests early coupling of transcription-translation
Sign Up to like & getrecommendations! Published in 2025 at "Nature Communications"
DOI: 10.1038/s41467-025-62207-w
Abstract: The main function of translation initiation factors is to assist ribosomes in selecting the correct reading frame on an mRNA. This process has been extensively studied with the help of reconstituted in vitro systems, but… read more here.
Keywords: if2 nusa; transcription translation; translation; translation complex ... See more keywords