Observing Single RNA Polymerase Molecules Down to Base-Pair Resolution.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-6421-5_15
Abstract: During transcriptional elongation, RNA polymerases (RNAP) employ a stepping mechanism to translocate along the DNA template while synthesizing RNA. Optical trapping assays permit the progress of single molecules of RNA polymerase to be monitored in… read more here.
Keywords: transcriptional elongation; rna polymerase; elongation; optical trapping ... See more keywords
Predictive model of transcriptional elongation control identifies trans regulatory factors from chromatin signatures
Sign Up to like & getrecommendations! Published in 2022 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkac1272
Abstract: Promoter-proximal Polymerase II (Pol II) pausing is a key rate limiting step for gene expression. DNA and RNA-binding trans-acting factors regulating the extent of pausing have been identified. However, we lack a quantitative model of… read more here.
Keywords: transcriptional elongation; promoter proximal; pol pausing; regulatory factors ... See more keywords
ICP22 of Herpes Simplex Virus 1 Decreases RNA Polymerase Processivity
Sign Up to like & getrecommendations! Published in 2022 at "Journal of Virology"
DOI: 10.1128/jvi.02191-21
Abstract: The longstanding observation that ICP22 reduces RNA polymerase II (Pol II) serine 2 phosphorylation, which initiates transcriptional elongation, is puzzling because this phosphorylation is essential for viral replication. The current study helps explain this apparent… read more here.
Keywords: polymerase; icp22; rna polymerase; transcriptional elongation ... See more keywords
Drosophila melanogaster positive transcriptional elongation factors regulate metabolic and sex-biased expression in adults
Sign Up to like & getrecommendations! Published in 2017 at "BMC Genomics"
DOI: 10.1186/s12864-017-3755-x
Abstract: BackgroundTranscriptional elongation is a generic function, but is also regulated to allow rapid transcription responses. Following relatively long initiation and promoter clearance, RNA polymerase II can pause and then rapidly elongate following recruitment of positive… read more here.
Keywords: elongation factors; transcriptional elongation; elongation; sex biased ... See more keywords
Nucleotide-level linkage of transcriptional elongation and polyadenylation
Sign Up to like & getrecommendations! Published in 2022 at "eLife"
DOI: 10.7554/elife.83153
Abstract: Alternative polyadenylation yields many mRNA isoforms whose 3’ termini occur disproportionately in clusters within 3’ UTRs. Previously, we showed that profiles of poly(A) site usage are regulated by the rate of transcriptional elongation by RNA… read more here.
Keywords: elongation; nucleotide level; transcriptional elongation; polyadenylation ... See more keywords