Constructing RNA Viruses for Long-Term Transcriptional Gene Silencing.
Sign Up to like & getrecommendations! Published in 2019 at "Trends in biotechnology"
DOI: 10.1016/j.tibtech.2018.07.015
Abstract: Recent discoveries have shown that self-replicating RNA viruses can produce small RNAs (sRNAs) in host cells. Given their potential to be modified to generate short-term transgene expression without integrating viral sequences into the host genome,… read more here.
Keywords: term; rna viruses; term transcriptional; long term ... See more keywords
Transgene‐independent heredity of RdDM‐mediated transcriptional gene silencing of endogenous genes in rice
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DOI: 10.1111/pbi.12934
Abstract: Summary To induce transcriptional gene silencing (TGS) of endogenous genes of rice (Oryza sativa L.), we expressed double‐strand RNA of each promoter region and thus induced RNA‐directed DNA methylation (RdDM). We targeted constitutively expressed genes… read more here.
Keywords: rice; tgs; heredity; transcriptional gene ... See more keywords
Transcriptional gene silencing in bread wheat (Triticum aestivum L.) and its application to regulate male fertility for hybrid seed production
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DOI: 10.1111/pbi.13895
Abstract: Summary Transcriptional gene silencing (TGS) can offer a straightforward tool for functional analysis of plant genes, particularly in polyploid species such as wheat, where genetic redundancy poses a challenge in applying mutagenesis approaches, including CRISPR… read more here.
Keywords: wheat; gene silencing; transcriptional gene; production ... See more keywords