Diphtheria toxin-mediated transposon-driven poly (A)-trapping efficiently disrupts transcriptionally silent genes in embryonic stem cells.
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DOI: 10.1002/dvg.23386
Abstract: Random gene trapping is the application of insertional mutagenesis techniques that are conventionally used to inactivate protein-coding genes in mouse embryonic stem (ES) cells. Transcriptionally silent genes are not effectively targeted by conventional random gene… read more here.
Keywords: vector; transcriptionally silent; embryonic stem; stem cells ... See more keywords
How Do Transposable Elements Activate Expression of Transcriptionally Silent Antibiotic Resistance Genes?
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DOI: 10.3390/ijms23158063
Abstract: The rapidly emerging phenomenon of antibiotic resistance threatens to substantially reduce the efficacy of available antibacterial therapies. Dissemination of resistance, even between phylogenetically distant bacterial species, is mediated mainly by mobile genetic elements, considered to… read more here.
Keywords: elements activate; antibiotic resistance; transposable elements; transcriptionally silent ... See more keywords