Multiplexed quantification of proteins and transcripts in single cells
Sign Up to like & getrecommendations! Published in 2017 at "Nature Biotechnology"
DOI: 10.1038/nbt.3973
Abstract: We present a tool to measure gene and protein expression levels in single cells with DNA-labeled antibodies and droplet microfluidics. Using the RNA expression and protein sequencing assay (REAP-seq), we quantified proteins with 82 barcoded… read more here.
Keywords: quantification proteins; proteins transcripts; multiplexed quantification; transcripts single ... See more keywords
Imaging and Quantification of mRNA Molecules at Single-Cell Resolution in the Human Fungal Pathogen Candida albicans
Sign Up to like & getrecommendations! Published in 2021 at "mSphere"
DOI: 10.1128/msphere.00411-21
Abstract: Tools to visualize and quantify transcripts at single-cell resolution have enabled the dissection of spatiotemporal patterns of gene expression in animal cells and tissues. Yet the accurate quantification of transcripts at single-cell resolution remains challenging… read more here.
Keywords: resolution; transcripts single; cell; single cell ... See more keywords