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Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1311-5_19
Abstract: Lentivirus-mediated gene transfer is an efficient method to introduce a variety of transgenes to human T cells. Here we describe a protocol to transduce human CD4+, CD8+, or CD4+ regulatory T cells. To illustrate the…
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Keywords:
cell subsets;
regulatory cells;
human cell;
cell ... See more keywords
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Published in 2022 at "Human gene therapy"
DOI: 10.1089/hum.2022.117
Abstract: Lentiviral transduction of human mesenchymal stem cells (MSCs) induces long-term transgene expression and holds great promise for multiple gene therapy applications. Polybrene is the most commonly used reagent to improve viral gene transfer efficiency in…
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Keywords:
transduction;
mesenchymal stem;
lentiviral transduction;
transduction human ... See more keywords