Induction of Bleb Structures in Lipid Nanoparticle Formulations of mRNA Leads to Improved Transfection Potency.
Sign Up to like & getrecommendations! Published in 2023 at "Advanced materials"
DOI: 10.1002/adma.202303370
Abstract: The transfection potency of lipid nanoparticle (LNP) mRNA systems is critically dependent on the ionizable cationic lipid component. LNP mRNA systems composed of optimized ionizable lipids often display distinctive mRNA rich "bleb" structures. Here we… read more here.
Keywords: improved transfection; transfection potency; potency; lipid nanoparticle ... See more keywords
Effective Cell Transfection in An Ultrasonically Levitated Droplet for Sustainable Technology
Sign Up to like & getrecommendations! Published in 2022 at "Advanced Science"
DOI: 10.1002/advs.202203576
Abstract: The levitation methodology, which enables us to operate a contactless reaction without a container, is likely to be a revolutionary technology in the fields of chemistry and biology to reduce the plastic waste in life… read more here.
Keywords: cell; technology; methodology; droplet ... See more keywords
Development of a petal protoplast transfection system for Sinningia speciosa
Sign Up to like & getrecommendations! Published in 2022 at "Applications in Plant Sciences"
DOI: 10.1002/aps3.11476
Abstract: Abstract Premise Transient gene expression systems are powerful tools for studying gene interactions in plant species without available or stable genetic transformation protocols. We optimized a petal protoplast transformation protocol for Sinningia speciosa, a model… read more here.
Keywords: system; sinningia speciosa; transfection system; petal protoplast ... See more keywords
A Branched Tripeptide with an Anion‐Binding Motif as a New Delivery Carrier for Efficient Gene Transfection
Sign Up to like & getrecommendations! Published in 2019 at "ChemBioChem"
DOI: 10.1002/cbic.201800728
Abstract: Branched and dendrimeric cationic peptides have shown better transfection efficiency than linear peptides, owing to their superior capacity for inducing DNA condensation. We have designed and synthesized two analogously guanidinocarbonylpyrrole‐substituted (GCP‐substituted) branched cationic tripeptides that… read more here.
Keywords: transfection; efficient gene; branched tripeptide; tripeptide anion ... See more keywords
Transfection reagent artefact likely accounts for some reports of extracellular vesicle function
Sign Up to like & getrecommendations! Published in 2022 at "Journal of Extracellular Vesicles"
DOI: 10.1002/jev2.12253
Abstract: Abstract Extracellular vesicles (EV) are important mediators of cell communication and physiology. EVs are frequently investigated by transiently transfecting cells with plasmid DNA to produce EVs modified with protein(s) or nucleic acid(s) of interest. DNA‐transfection… read more here.
Keywords: function; dna; plasmid dna; transfection reagent ... See more keywords
Construction of Bovine Serum Albumin/AIE-Based Quaternary Complexes for Efficient Gene Transfection.
Sign Up to like & getrecommendations! Published in 2019 at "Macromolecular bioscience"
DOI: 10.1002/mabi.201800359
Abstract: High transfection efficiency and superior cell imaging are required for cationic polymers-based gene delivery system to afford high therapeutic effect but its high toxicity and unstable cell imaging are easily ignored. In this study, cationic… read more here.
Keywords: transfection; bsa; gene transfection; gene ... See more keywords
Improvement of Transfection with PepFects Using Organic and Inorganic Materials.
Sign Up to like & getrecommendations! Published in 2022 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1752-6_35
Abstract: Cell-penetrating peptides (CPPs) are a promising non-viral vector for gene and drug delivery. CPPs exhibit high cell transfection, and are biocompatible. They can be also conjugated with organic and inorganic nanomaterials, such as magnetic nanoparticles… read more here.
Keywords: transfection; organic inorganic; cell transfection; high cell ... See more keywords
Large-Scale Transient Transfection of Chinese Hamster Ovary Cells in Suspension.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-6972-2_3
Abstract: We describe a one-liter transfection of suspension-adapted Chinese hamster ovary (CHO-DG44) cells using polyethyleneimine (PEI) for DNA delivery. The method involves transfection at a high cell density (5 × 106 cells/mL) by direct addition of… read more here.
Keywords: large scale; transfection; suspension; hamster ovary ... See more keywords
Stable Expression of Epigenome Editors via Viral Delivery and Genomic Integration.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7774-1_11
Abstract: The advent of precise genomic targeting systems has revolutionized epigenome editing through fusion of epigenetic effector proteins with engineered DNA-binding proteins. However, the delivery of plasmid DNA to express these fusion proteins via conventional transient… read more here.
Keywords: transfection; delivery; expression; fusion ... See more keywords
Microfluidic Device for Localized Electroporation.
Sign Up to like & getrecommendations! Published in 2020 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9740-4_10
Abstract: Electroporation is a common method of transfection due to its relatively low risk and high transfection efficiency. The most common method of electroporation is bulk electroporation which is easily performed on large quantities of cells… read more here.
Keywords: transfection; device localized; localized electroporation; electroporation ... See more keywords
Transfection of primary brain capillary endothelial cells for protein synthesis and secretion of recombinant erythropoietin: a strategy to enable protein delivery to the brain
Sign Up to like & getrecommendations! Published in 2017 at "Cellular and Molecular Life Sciences"
DOI: 10.1007/s00018-017-2501-5
Abstract: Treatment of chronic disorders affecting the central nervous system (CNS) is complicated by the inability of drugs to cross the blood–brain barrier (BBB). Non-viral gene therapy applied to brain capillary endothelial cells (BCECs) denotes a… read more here.
Keywords: protein; secretion; transfection; bbb ... See more keywords