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   Articles with "transfection" as a keyword



Induction of Bleb Structures in Lipid Nanoparticle Formulations of mRNA Leads to Improved Transfection Potency.

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recommendations! Published in 2023 at "Advanced materials"

DOI: 10.1002/adma.202303370

Abstract: The transfection potency of lipid nanoparticle (LNP) mRNA systems is critically dependent on the ionizable cationic lipid component. LNP mRNA systems composed of optimized ionizable lipids often display distinctive mRNA rich "bleb" structures. Here we… read more here.

Keywords: improved transfection; transfection potency; potency; lipid nanoparticle ... See more keywords

Effective Cell Transfection in An Ultrasonically Levitated Droplet for Sustainable Technology

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recommendations! Published in 2022 at "Advanced Science"

DOI: 10.1002/advs.202203576

Abstract: The levitation methodology, which enables us to operate a contactless reaction without a container, is likely to be a revolutionary technology in the fields of chemistry and biology to reduce the plastic waste in life… read more here.

Keywords: cell; technology; methodology; droplet ... See more keywords

mRNA Sonotransfection of Tumors with Polymeric Microbubbles: Co‐Formulation versus Co‐Administration

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recommendations! Published in 2024 at "Advanced Science"

DOI: 10.1002/advs.202306139

Abstract: Despite its high potential, non‐viral gene therapy of cancer remains challenging due to inefficient nucleic acid delivery. Ultrasound (US) with microbubbles (MB) can open biological barriers and thus improve DNA and mRNA passage. Polymeric MB… read more here.

Keywords: formulation; transfection; mrna sonotransfection; administration ... See more keywords

ROS‐sensitive selenium‐containing cationic brush polymer with potent gene transfection efficiency and biocompatibility

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recommendations! Published in 2024 at "Journal of Applied Polymer Science"

DOI: 10.1002/app.55199

Abstract: Smart gene delivery vectors are gaining increasing attention in gene therapy, owing to their low cytotoxicity and intrinsic responsiveness. Our previously fabricated novel cationic brush polymer, comprising CSe bonds and tertiary amine EGIn‐g‐PDMAEMA, shows potential… read more here.

Keywords: efficiency; transfection; brush polymer; polymer ... See more keywords

Development of a petal protoplast transfection system for Sinningia speciosa

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recommendations! Published in 2022 at "Applications in Plant Sciences"

DOI: 10.1002/aps3.11476

Abstract: Abstract Premise Transient gene expression systems are powerful tools for studying gene interactions in plant species without available or stable genetic transformation protocols. We optimized a petal protoplast transformation protocol for Sinningia speciosa, a model… read more here.

Keywords: system; sinningia speciosa; transfection system; petal protoplast ... See more keywords

Multidose transient transfection of human embryonic kidney 293 cells modulates recombinant adeno‐associated virus2/5 Rep protein expression and influences the enrichment fraction of filled capsids

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recommendations! Published in 2024 at "Biotechnology and Bioengineering"

DOI: 10.1002/bit.28828

Abstract: Recombinant adeno‐associated virus (rAAV) is a commonly used in vivo gene therapy vector because of its nonpathogenicity, long‐term transgene expression, broad tropism, and ability to transduce both dividing and nondividing cells. However, rAAV vector production… read more here.

Keywords: recombinant adeno; capsid; transfection; expression ... See more keywords

An Overview of Virus‐Free Protein Expression in Insect Cells: A Mode of Rapid Manufacturing Platform for Therapeutic Protein and Virus‐Like‐Particles

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recommendations! Published in 2025 at "Biotechnology and Bioengineering"

DOI: 10.1002/bit.28961

Abstract: Production of therapeutic proteins, antibodies, and virus‐like particles (VLP) using baculovirus expression systems (BEVS) has been explored for decades. However, we have realized an urgent need for accelerated production of recombinant proteins and VLPs to… read more here.

Keywords: transfection; protein; virus like; expression ... See more keywords

High‐yield recombinant adeno‐associated viral vector production by multivariate optimization of bioprocess and transfection conditions

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recommendations! Published in 2024 at "Biotechnology Progress"

DOI: 10.1002/btpr.3445

Abstract: Recombinant adeno‐associated viral vectors (rAAVs) are one of the most used vehicles for gene therapy, with five rAAV therapeutics commercially approved by the FDA. To improve product yield, we optimized the suspension production process of… read more here.

Keywords: multivariate; recombinant adeno; vector; yield ... See more keywords

A Branched Tripeptide with an Anion‐Binding Motif as a New Delivery Carrier for Efficient Gene Transfection

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recommendations! Published in 2019 at "ChemBioChem"

DOI: 10.1002/cbic.201800728

Abstract: Branched and dendrimeric cationic peptides have shown better transfection efficiency than linear peptides, owing to their superior capacity for inducing DNA condensation. We have designed and synthesized two analogously guanidinocarbonylpyrrole‐substituted (GCP‐substituted) branched cationic tripeptides that… read more here.

Keywords: transfection; efficient gene; branched tripeptide; tripeptide anion ... See more keywords

Transfection reagent artefact likely accounts for some reports of extracellular vesicle function

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recommendations! Published in 2022 at "Journal of Extracellular Vesicles"

DOI: 10.1002/jev2.12253

Abstract: Abstract Extracellular vesicles (EV) are important mediators of cell communication and physiology. EVs are frequently investigated by transiently transfecting cells with plasmid DNA to produce EVs modified with protein(s) or nucleic acid(s) of interest. DNA‐transfection… read more here.

Keywords: function; dna; plasmid dna; transfection reagent ... See more keywords

Construction of Bovine Serum Albumin/AIE-Based Quaternary Complexes for Efficient Gene Transfection.

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recommendations! Published in 2019 at "Macromolecular bioscience"

DOI: 10.1002/mabi.201800359

Abstract: High transfection efficiency and superior cell imaging are required for cationic polymers-based gene delivery system to afford high therapeutic effect but its high toxicity and unstable cell imaging are easily ignored. In this study, cationic… read more here.

Keywords: transfection; bsa; gene transfection; gene ... See more keywords