Synthesis of Double hydrophilic block copolymers poly(2-isopropyl-2-oxazoline-b-ethylenimine) and their DNA transfection efficiency.
Sign Up to like & getrecommendations! Published in 2022 at "Macromolecular bioscience"
DOI: 10.1002/mabi.202200296
Abstract: Gene delivery is now a part of our therapeutic arsenal for vaccination and treatments of inherited or acquired diseases. Polymers represent an opportunity to develop new synthetic vectors for gene transfer, with a prerequisite of… read more here.
Keywords: dna; piprox; block copolymers; block ... See more keywords
A method for high transfection efficiency in THP-1 suspension cells without PMA treatment.
Sign Up to like & getrecommendations! Published in 2018 at "Analytical biochemistry"
DOI: 10.1016/j.ab.2017.12.032
Abstract: Adherent cells such as mouse RAW cells or human cancer U2OS cells are beneficial to DNA transfection, with 20%-60% transfection efficiency. However, this DNA transfection is rarely used on suspension cells due to its low… read more here.
Keywords: transfection efficiency; suspension cells; transfection; method ... See more keywords
Cell-based high-throughput screening of cationic polymers for efficient DNA and siRNA delivery.
Sign Up to like & getrecommendations! Published in 2020 at "Acta biomaterialia"
DOI: 10.1016/j.actbio.2020.08.029
Abstract: Development of non-viral gene vectors which can efficiently and safely transfect plasmid DNA and siRNA into cells is of great importance for gene therapy. Despite lots of efforts spent, it is still imperative to develop… read more here.
Keywords: dna sirna; gene; transfection efficiency; transfection ... See more keywords
Control of the transfection efficiency of human dermal fibroblasts by adjusting the characteristics of jetPEI®/plasmid complexes/polyplexes through the cation/anion ratio
Sign Up to like & getrecommendations! Published in 2018 at "Colloids and Surfaces A: Physicochemical and Engineering Aspects"
DOI: 10.1016/j.colsurfa.2018.04.035
Abstract: Abstract Primary cells are in general more difficult to transfect than most animal cells. Here we report on the efficient transfection of human dermal fibroblasts with jetPEI®/DNA complexes (polyplexes). The polycation/DNA ratio was varied with… read more here.
Keywords: transfection efficiency; transfection; dna; ratio ... See more keywords
Plasmid transfection in bovine cells: Optimization using a realtime monitoring of green fluorescent protein and effect on gene reporter assay.
Sign Up to like & getrecommendations! Published in 2017 at "Gene"
DOI: 10.1016/j.gene.2017.05.025
Abstract: Gene reporter technology (GRT) has opened several new avenues for monitoring biological events including the activation of transcription factors, which are central to the study of nutrigenomics. However, this technology relies heavily on the insertion… read more here.
Keywords: bovine; transfection efficiency; mdbk cells; transfection ... See more keywords
Novel chitosan based nanoparticles as gene delivery systems to cancerous and noncancerous cells
Sign Up to like & getrecommendations! Published in 2019 at "International Journal of Pharmaceutics"
DOI: 10.1016/j.ijpharm.2019.02.016
Abstract: &NA; The present study aimed to investigate in vitro DNA transfection efficiency of three novel chitosan derivatives: thiolated trimethyl chitosan (TMC‐Cys), methylated 4‐N,N dimethyl aminobenzyl N,O carboxymethyl chitosan(MABCC) and thiolated trimethyl aminobenzyl chitosan(MABC‐Cys). After polymer… read more here.
Keywords: cys; gene delivery; novel chitosan; transfection efficiency ... See more keywords
Cell Penetrating Polymers Containing Guanidinium Trigger Apoptosis in Human Hepatocellular Carcinoma Cells unless Conjugated to a Targeting N-Acetyl-Galactosamine Block.
Sign Up to like & getrecommendations! Published in 2017 at "Bioconjugate chemistry"
DOI: 10.1021/acs.bioconjchem.7b00598
Abstract: A series of 3-guanidinopropyl methacrylamide (GPMA)-based polymeric gene delivery vehicles were developed via aqueous reversible addition-fragmentation chain transfer (RAFT) polymerization. The polymers have been evaluated for their cellular internalization ability, transfection efficiency, and cytotoxicity. Two… read more here.
Keywords: transfection efficiency; block; acetyl galactosamine; human hepatocellular ... See more keywords
Synthetic Helical Polypeptide as a Gene Transfection Enhancer.
Sign Up to like & getrecommendations! Published in 2022 at "Biomacromolecules"
DOI: 10.1021/acs.biomac.2c00331
Abstract: The relatively low transfection efficiency limits further application of polymeric gene carriers. It is imperative to exploit a universal and simple strategy to enhance the gene transfection efficiency of polymeric gene carriers. Herein, we prepared… read more here.
Keywords: polymeric gene; gene transfection; transfection efficiency; gene ... See more keywords
Polypeptide Bilayer Assembly-Mediated Gene Delivery Enhances Chemotherapy in Cancer Cells.
Sign Up to like & getrecommendations! Published in 2022 at "Molecular pharmaceutics"
DOI: 10.1021/acs.molpharmaceut.2c00861
Abstract: Developing gene vectors with high transfection efficiency and low cytotoxicity to humans is crucial to improve gene therapy outcomes. This study set out to investigate the use of cationic polypeptide bilayer assemblies formed by coil-sheet… read more here.
Keywords: polypeptide bilayer; bilayer; transfection efficiency; gene ... See more keywords
Effect of the array of amines on the transfection efficiency of cationic peptidomimetic lipid molecules into neural cells
Sign Up to like & getrecommendations! Published in 2022 at "RSC Advances"
DOI: 10.1039/d2ra03347j
Abstract: The amino groups in the head group of a cationic lipid play a determinative role regarding the nucleic acid delivery efficiency of the LNP formulated from lipids. Herein, we designed four types of lipid bearing… read more here.
Keywords: neural cells; denoted lipid; transfection efficiency; efficiency ... See more keywords
Small Molecule Modifications Significantly Increase the Transfection Efficiency of Low-Molecular Polymer.
Sign Up to like & getrecommendations! Published in 2022 at "Journal of biomedical nanotechnology"
DOI: 10.1166/jbn.2022.3252
Abstract: Gene vectors with high biocompatibility and transfection efficiency are critical for successful gene therapy. PEI 25K (Polyethyleneimine 25K) is a common polymeric gene vector that has been employed as a positive control material in gene… read more here.
Keywords: small molecule; transfection efficiency; gene; low molecular ... See more keywords