Enhancing the Heterologous Fructosyltransferase Activity of Kluyveromyces lactis: Developing a Scaled-Up Process and Abolishing Invertase by CRISPR/Cas9 Genome Editing
Sign Up to like & getrecommendations! Published in 2020 at "Frontiers in Bioengineering and Biotechnology"
DOI: 10.3389/fbioe.2020.607507
Abstract: The enzymatic production of prebiotic fructo-oligosaccharides (FOS) from sucrose involves fructosyltransferases (FFTs) and invertases, both of which catalyze forward (transferase) and reverse (hydrolysis) reactions. FOS yields can therefore be increased by favoring the forward reaction.… read more here.
Keywords: invertase; scaled process; kluyveromyces lactis; transferase activity ... See more keywords
Key Amino Acids for Transferase Activity of GDSL Lipases
Sign Up to like & getrecommendations! Published in 2022 at "International Journal of Molecular Sciences"
DOI: 10.3390/ijms232315141
Abstract: The Gly-Asp-Ser-Leu (GDSL) motif of esterase/lipase family proteins (GELPs) generally exhibit esterase activity, whereas transferase activity is markedly preferred in several GELPs, including the Tanacetum cinerariifolium GDSL lipase TciGLIP, which is responsible for the biosynthesis… read more here.
Keywords: amino acids; key amino; activity; transferase activity ... See more keywords