Yeast transformation efficiency is enhanced by TORC1‐ and eisosome‐dependent signaling
Sign Up to like & getrecommendations! Published in 2019 at "MicrobiologyOpen"
DOI: 10.1002/mbo3.730
Abstract: Transformation of baker's yeast (Saccharomyces cerevisiae) plays a key role in several experimental techniques, yet the molecular mechanisms underpinning transformation are still unclear. The addition of amino acids to the growth and transformation medium increases… read more here.
Keywords: enhanced torc1; transformation efficiency; efficiency enhanced; transformation ... See more keywords
Small-Molecule Screening to Increase Agrobacterium-Mediated Transformation Efficiency in Legumes.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7874-8_8
Abstract: Agrobacterium-mediated transformation is a powerful strategy for plant genetic engineering. However, the transformation efficiency of some grain legume crops is generally low. In this chapter, we describe a chemical screening procedure for improving the efficiency… read more here.
Keywords: mediated transformation; transformation efficiency; agrobacterium mediated; transformation ... See more keywords
Political incentives, transformation efficiency and resource-exhausted cities
Sign Up to like & getrecommendations! Published in 2018 at "Journal of Cleaner Production"
DOI: 10.1016/j.jclepro.2018.06.093
Abstract: Abstract Since 2003, the evaluation system of government officials in China has undergone significant changes from relying on almost solely on traditional economic growth criteria to a broader set of indicators including environmental quality and… read more here.
Keywords: transformation efficiency; resource exhausted; exhausted cities; transformation ... See more keywords
Bioaccumulation and detoxification of trivalent arsenic by Achromobacter xylosoxidans BHW-15 and electrochemical detection of its transformation efficiency
Sign Up to like & getrecommendations! Published in 2021 at "Scientific Reports"
DOI: 10.1038/s41598-021-00745-1
Abstract: Arsenotrophic bacteria play an essential role in lowering arsenic contamination by converting toxic arsenite [As (III)] to less toxic and less bio-accumulative arsenate [As (V)]. The current study focused on the qualitative and electrocatalytic detection… read more here.
Keywords: detection; transformation efficiency; detoxification; xylosoxidans bhw ... See more keywords
Deletion of biofilm synthesis in Eubacterium limosum ATCC 8486 improves handling and transformation efficiency.
Sign Up to like & getrecommendations! Published in 2023 at "FEMS microbiology letters"
DOI: 10.1093/femsle/fnad030
Abstract: Eubacterium limosum is an acetogenic bacterium of potential industrial relevance for its ability to efficiently metabolize a range of single carbon compounds. However, extracellular polymeric substance (EPS) produced by the type strain ATCC 8486 is… read more here.
Keywords: transformation efficiency; atcc 8486; eubacterium limosum;
The function of the gene loiP in transformation efficiency and outer membrane permeability change of Escherichia coli treated by Ca2+ ions.
Sign Up to like & getrecommendations! Published in 2023 at "Letters in applied microbiology"
DOI: 10.1093/lambio/ovac076
Abstract: The preparation of Escherichia coli competent cells by calcium chloride is a common method in molecular biology, but the mechanism is poorly understood. In a previous study, using transcriptomics and proteomics approaches, we found that… read more here.
Keywords: transformation efficiency; outer membrane; gene loip; permeability ... See more keywords
An Optimized Transformation Protocol for Escherichia coli BW3KD with Supreme DNA Assembly Efficiency
Sign Up to like & getrecommendations! Published in 2022 at "Microbiology Spectrum"
DOI: 10.1128/spectrum.02497-22
Abstract: DNA transformation is commonly used in cloning; however, high transformation efficiency becomes a limiting factor in many applications, such as the construction of CRISPR and DNA libraries, the assembly of multiple fragments, and the transformation… read more here.
Keywords: transformation efficiency; competent; dna; bw3kd ... See more keywords
Succinate, iron chelation, and monovalent cations affect the transformation efficiency of Acinetobacter baylyi ATCC 33305 during growth in complex media.
Sign Up to like & getrecommendations! Published in 2017 at "Canadian journal of microbiology"
DOI: 10.1139/cjm-2017-0393
Abstract: Natural transformation is the acquisition of new genetic material via the uptake of exogenous DNA by competent bacteria. Acinetobacter baylyi is model for natural transformation. Here we focus on the natural transformation of A. baylyi… read more here.
Keywords: acinetobacter baylyi; transformation efficiency; natural transformation; transformation ... See more keywords
White LED intensities during co-cultivation affect the Agrobacterium-mediated soybean (Glycine max) transformation using mature half seeds as explants
Sign Up to like & getrecommendations! Published in 2024 at "PLOS ONE"
DOI: 10.1371/journal.pone.0312129
Abstract: The transition of light fixture from fluorescent light to light-emitting diodes (LEDs) in growth chambers prompts a reevaluation of current practices in plant biotechnology. Agrobacterium-mediated transformation is crucial for genetic engineering and genome editing in… read more here.
Keywords: soybean glycine; cultivation; transformation efficiency; agrobacterium mediated ... See more keywords
Green Transformation Efficiency and Its Influencing Factors in Resource-Based Cities: A Case of The Yellow River Basin in China
Sign Up to like & getrecommendations! Published in 2024 at "Polish Journal of Environmental Studies"
DOI: 10.15244/pjoes/185338
Abstract: Reasonably assessing green transformation efficiency (GTE) and clarifying influencing factors can provide theoretical support for their sustainable development. This study utilizes the undesired super-efficient Slack Based Measure model (SE-SBM), window analysis, kernel density estimation analysis,… read more here.
Keywords: resource based; green transformation; level; influencing factors ... See more keywords
GhLBDs Promote Callus Initiation and Act as Selectable Markers to Increase Transformation Efficiency
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Plant Science"
DOI: 10.3389/fpls.2022.861706
Abstract: Detached organs or differentiated tissues could form a mass of pluripotent cells termed as callus on an auxin-rich medium, the underlying molecular mechanism of which remains elusive in cotton. LATERAL ORGAN BOUNDARIES DOMAIN (LBD) transcription… read more here.
Keywords: expression; transformation efficiency; selectable markers; callus initiation ... See more keywords