Insights into hydrolysis versus transfructosylation: Mutagenesis studies of a novel levansucrase from Brenneria sp. EniD312.
Sign Up to like & getrecommendations! Published in 2018 at "International journal of biological macromolecules"
DOI: 10.1016/j.ijbiomac.2018.05.029
Abstract: Levan is a kind of fructan that composing of fructose by β-(2, 6) linkage and has been already applied as thickening agent and colloidal stabilizer in the cosmetic, medicinal and food industries. Microbial levansucrase is… read more here.
Keywords: levansucrase brenneria; transfructosylation; brenneria enid312; mutagenesis ... See more keywords
Impaired coordination of nucleophile and increased hydrophobicity in the +1 subsite shift levansucrase activity towards transfructosylation
Sign Up to like & getrecommendations! Published in 2017 at "Glycobiology"
DOI: 10.1093/glycob/cwx050
Abstract: Abstract Bacterial levansucrases produce β(2,6)-linked levan-type polysaccharides using sucrose or sucrose analogs as donor/acceptor substrates. However, the dominant reaction of Bacillus megaterium levansucrase (Bm-LS) is hydrolysis. Single domain levansucrases from Gram-positive bacteria display a wide… read more here.
Keywords: coordination nucleophile; levansucrase; transfructosylation; water ... See more keywords