Assessment of resistance to rice tungro disease in popular rice varieties in India by introgression of a transgene against Rice tungro bacilliform virus
Sign Up to like & getrecommendations! Published in 2019 at "Archives of Virology"
DOI: 10.1007/s00705-019-04159-3
Abstract: Rice crops in South and Southeast Asian countries suffer critical yield losses due to rice tungro disease caused by joint infection with rice tungro bacilliform virus (RTBV) and rice tungro spherical virus (RTSV). Previously, for… read more here.
Keywords: tungro; rice; resistance; transgene ... See more keywords
A murine Ig light chain transgene reveals IGKV3 gene contributions to anti‐collagen types IV and II specificities
Sign Up to like & getrecommendations! Published in 2017 at "Molecular Immunology"
DOI: 10.1016/j.molimm.2017.08.015
Abstract: HighlightsIGKV3‐encoded light chains are prominent among murine anti‐type II and anti‐type IV collagen autoantibodies.Mice bearing an IGKV3 light chain transgene develop subsets of B cells reactive with each collagen.IGKV3 subgroup genes contribute structural fitness to… read more here.
Keywords: collagen; igkv3; anti collagen; light chain ... See more keywords
Improvements of TKC Technology Accelerate Isolation of Transgene-Free CRISPR/Cas9-Edited Rice Plants
Sign Up to like & getrecommendations! Published in 2019 at "Rice Science"
DOI: 10.1016/j.rsci.2018.11.001
Abstract: Abstract Elimination of the CRISPR/Cas9 constructs in edited plants is a prerequisite for assessing genetic stability, conducting phenotypic characterization, and applying for commercialization of the plants. However, removal of the CRISPR/Cas9 transgenes by genetic segregation… read more here.
Keywords: rice; crispr; tkc technology; transgene ... See more keywords
In utero delivery of rAAV2/9 induces neuronal expression of the transgene in the brain: towards new models of Parkinson’s disease
Sign Up to like & getrecommendations! Published in 2017 at "Gene Therapy"
DOI: 10.1038/gt.2017.84
Abstract: Animal models are essential tools for basic pathophysiological research as well as validation of therapeutic strategies for curing human diseases. However, technical difficulties associated with classical transgenesis approaches in rodent species higher than Mus musculus… read more here.
Keywords: delivery; expression; utero delivery; transgene ... See more keywords
Replicating minichromosomes as a new tool for plastid genome engineering.
Sign Up to like & getrecommendations! Published in 2021 at "Nature plants"
DOI: 10.1038/s41477-021-00940-y
Abstract: Plant molecular farming, that is, using plants as hosts for production of therapeutic proteins and high-value compounds, has gained substantial interest in recent years. Chloroplasts in particular are an attractive subcellular compartment for expression of… read more here.
Keywords: tool; replicating minichromosomes; expression; transgene ... See more keywords
Blastocyst Formation Rate and Transgene Expression are Associated with Gene Insertion into Safe and Non-Safe Harbors in the Cattle Genome
Sign Up to like & getrecommendations! Published in 2017 at "Scientific Reports"
DOI: 10.1038/s41598-017-15648-3
Abstract: Integration target site is the most important factor in successful production of transgenic animals. However, stable expression of transgene without disturbing the function of the host genome depends on promoter methylation, transgene copy number and… read more here.
Keywords: non safe; expression; rate; safe non ... See more keywords
Mesenchymal Stromal Cells in Bone Marrow Express Adiponectin and Are Efficiently Targeted by an Adiponectin Promoter-Driven Cre Transgene.
Sign Up to like & getrecommendations! Published in 2019 at "International immunology"
DOI: 10.1093/intimm/dxz042
Abstract: Stromal cells in bone marrow (BM) constitute a specific microenvironment supporting development and maintenance of hematopoietic cells. Adiponectin is a cytokine secreted by adipocytes. Besides its anti-diabetic and anti-atherogenic roles, adiponectin reportedly regulates development and… read more here.
Keywords: express adiponectin; stromal cells; transgene; cre transgene ... See more keywords
Adaptation of transgene mRNA translation boosts the anticancer efficacy of oncolytic HSV1
Sign Up to like & getrecommendations! Published in 2023 at "Journal for Immunotherapy of Cancer"
DOI: 10.1136/jitc-2022-006408
Abstract: Background Transgenes deliver therapeutic payloads to improve oncolytic virus immunotherapy. Transgenes encoded within oncolytic viruses are designed to be highly transcribed, but protein synthesis is often negatively affected by viral infection, compromising the amount of… read more here.
Keywords: hsv1; leader; transgene; oncolytic hsv1 ... See more keywords
Abstract CT237: Evaluation of in vivo chimeric antigen receptor (CAR) transgene levels in patients (pts) treated with tisagenlecleucel
Sign Up to like & getrecommendations! Published in 2019 at "Clinical Trials"
DOI: 10.1158/1538-7445.am2019-ct237
Abstract: Background: Following infusion of tisagenlecleucel, quantitative polymerase chain reaction (qPCR) can be used to measure in vivo kinetics of CAR transgene. The utility of qPCR to inform individual pt treatment decisions and measure functional persistence… read more here.
Keywords: evaluation vivo; transgene levels; car transgene; transgene ... See more keywords
Effect of transcription terminator usage on the establishment of transgene transcriptional gene silencing
Sign Up to like & getrecommendations! Published in 2018 at "BMC Research Notes"
DOI: 10.1186/s13104-018-3649-2
Abstract: ObjectiveObtaining high and stable transgene expression is of vital importance for plant genetic engineering. A lot is known about the relationship between terminator efficiency and gene expression, but no studies have addressed the relationship between… read more here.
Keywords: terminator usage; terminator; transgene; gene ... See more keywords
A polycistronic transgene design for combinatorial genetic perturbations from a single transcript in Drosophila.
Sign Up to like & getrecommendations! Published in 2023 at "PLoS genetics"
DOI: 10.1371/journal.pgen.1010792
Abstract: Experimental models that capture the genetic complexity of human disease and allow mechanistic explorations of the underlying cell, tissue, and organ interactions are crucial to furthering our understanding of disease biology. Such models require combinatorial… read more here.
Keywords: genetic perturbations; disease; drosophila; design ... See more keywords