Engineering Chloroplasts for High-Level Constitutive or Inducible Transgene Expression.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1472-3_3
Abstract: Expression of transgenes from the plastid genome offers a number of attractions to biotechnologists, with the potential to attain very high protein accumulation levels arguably being the most attractive one. High-level transgene expression is of… read more here.
Keywords: high level; expression; engineering chloroplasts; transgene expression ... See more keywords
ISSLS prize in basic science 2021: a novel inducible system to regulate transgene expression of TIMP1
Sign Up to like & getrecommendations! Published in 2021 at "European Spine Journal"
DOI: 10.1007/s00586-021-06728-0
Abstract: Inflammatory and oxidative stress upregulates matrix metalloproteinase (MMP) activity, leading to intervertebral disc degeneration (IDD). Gene therapy using human tissue inhibitor of metalloproteinase 1 (hTIMP1) has effectively treated IDD in animal models. However, persistent unregulated… read more here.
Keywords: transgene expression; htimp1 cells; aav htimp1; htimp1 ... See more keywords
Reactivation of rolB transgene expression in Vitis amurensis Rupr. cells upon retransformation with 2b gene from Cucumovirus isolate NK
Sign Up to like & getrecommendations! Published in 2018 at "Biotechnology Letters"
DOI: 10.1007/s10529-018-02642-6
Abstract: ObjectiveStudies concerning 2b protein from Cucumovirus showed 2b to effectively repress functioning of the plant silencing complex, current study aimed whether retransformation with 2b gene able to restore silenced transgene expression in plant cells.ResultsA rolB-transgenic… read more here.
Keywords: transgene expression; rolb; cucumovirus; expression ... See more keywords
Delivery of Anti-IFNAR1 shRNA to Hepatic Cells Decreases IFNAR1 Gene Expression and Improves Adenoviral Transduction and Transgene Expression
Sign Up to like & getrecommendations! Published in 2021 at "Molecular Biotechnology"
DOI: 10.1007/s12033-021-00408-6
Abstract: Chronic liver injury leads to advanced fibrosis, cirrhosis, and hepatocellular carcinoma. Genetical cell treatment related to the use of adenovirus (Ads) has proven to be beneficial and efficient in the recovery of hepatic diseases. Nevertheless,… read more here.
Keywords: ifnar1; transgene expression; gene expression; expression ... See more keywords
AAV6 as an effective gene delivery vector for prolonged transgene expression in intervertebral disc cells in vivo
Sign Up to like & getrecommendations! Published in 2020 at "Genes and Diseases"
DOI: 10.1016/j.gendis.2020.12.009
Abstract: Abstract Intervertebral disc degeneration is the main contributor to low back pain, now the leading cause of disability worldwide. Gene transfer, either in a therapeutic attempt or in basic research to understand the mechanisms of… read more here.
Keywords: cells vivo; transgene expression; delivery; intervertebral disc ... See more keywords
Mitochondrial transgene expression via an artificial mitochondrial DNA vector in cells from a patient with a mitochondrial disease
Sign Up to like & getrecommendations! Published in 2018 at "Journal of Controlled Release"
DOI: 10.1016/j.jconrel.2018.02.005
Abstract: &NA; To achieve mitochondrial gene therapy, developing a mitochondrial transgene expression system that produces therapeutic proteins in mitochondria of disease cells is essential. We previously reported on the design of pCMV‐mtLuc (CGG) containing a CMV… read more here.
Keywords: mtluc cgg; transgene expression; pcmv mtluc; expression ... See more keywords
Development and in vitro evaluation of recombinant chicken promoters to efficiently drive transgene expression in chicken oviduct cells
Sign Up to like & getrecommendations! Published in 2021 at "Poultry Science"
DOI: 10.1016/j.psj.2021.101365
Abstract: Virus injection into EGK-X embryos is a well-defined approach in avian transgenesis. This system uses a chicken ovalbumin gene promoter to induce transgene expression in the chicken oviduct. Although a reconstructed chicken ovalbumin promoter that… read more here.
Keywords: ovalbumin promoter; transgene expression; promoter; chicken ... See more keywords
Detailed comparison of retroviral vectors and promoter configurations for stable and high transgene expression in human induced pluripotent stem cells
Sign Up to like & getrecommendations! Published in 2017 at "Gene Therapy"
DOI: 10.1038/gt.2017.20
Abstract: Correction of patient-specific induced pluripotent stem cells (iPSC) upon gene delivery through retroviral vectors offers new treatment perspectives for monogenetic diseases. Gene-modified iPSC clones can be screened for safe integration sites and differentiated into transplantable… read more here.
Keywords: transgene expression; retroviral vectors; expression; pluripotent stem ... See more keywords
Benefits of using genomic insulators flanking transgenes to increase expression and avoid positional effects
Sign Up to like & getrecommendations! Published in 2019 at "Scientific Reports"
DOI: 10.1038/s41598-019-44836-6
Abstract: For more than 20 years, plant biologists have tried to achieve complete control of transgene expression. Until the techniques to target transgenes to safe harbor sites in the genome become routine, flanking transgenes with genetic… read more here.
Keywords: transgene expression; avoid positional; flanking transgenes; benefits using ... See more keywords
Matrix attachment region combinations increase transgene expression in transfected Chinese hamster ovary cells
Sign Up to like & getrecommendations! Published in 2017 at "Scientific Reports"
DOI: 10.1038/srep42805
Abstract: Matrix attachment regions (MARs) are cis-acting DNA elements that can increase transgene expression levels in a CHO cell expression system. To investigate the effects of MAR combinations on transgene expression and the underlying regulatory mechanisms,… read more here.
Keywords: transgene expression; expression; increase transgene; attachment region ... See more keywords
Effective intraperitoneal gene transfection system using nanobubbles and ultrasound irradiation
Sign Up to like & getrecommendations! Published in 2017 at "Drug Delivery"
DOI: 10.1080/10717544.2017.1319433
Abstract: Abstract In this study, we demonstrate the low toxicity and highly efficient and spatially improved transfection of plasmid DNA (pDNA) with liposomal nanobubbles (bubble liposomes [BLs]) using ultrasound (US) irradiation in mice. Naked pDNA with… read more here.
Keywords: ultrasound irradiation; irradiation; transfection; transgene expression ... See more keywords