Transgene insertion into the plastid genome alters expression of adjacent native chloroplast genes at the transcriptional and translational levels
Sign Up to like & getrecommendations! Published in 2022 at "Plant Biotechnology Journal"
DOI: 10.1111/pbi.13985
Abstract: Summary In plant biotechnology and basic research, chloroplasts have been used as chassis for the expression of various transgenes. However, potential unintended side effects of transgene insertion and highâlevel transgene expression on the expression of… read more here.
Keywords: native chloroplast; chloroplast; transgene insertion; expression ... See more keywords
Analysis of the transgene insertion pattern in a transgenic mouse strain using long-read sequencing
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DOI: 10.1538/expanim.19-0118
Abstract: Transgene insertion patterns are critical for the analysis of transgenic animals because the influence of transgenes may change depending on the insertion pattern (such as copy numbers and orientations of concatenations) and the insertion position… read more here.
Keywords: long read; insertion; analysis; transgene insertion ... See more keywords