LIFE‐Seq: a universal Large Integrated DNA Fragment Enrichment Sequencing strategy for deciphering the transgene integration of genetically modified organisms
Sign Up to like & getrecommendations! Published in 2022 at "Plant Biotechnology Journal"
DOI: 10.1111/pbi.13776
Abstract: Summary Molecular characterization of genetically modified organisms (GMOs) yields basic information on exogenous DNA integration, including integration sites, entire inserted sequences and structures, flanking sequences and copy number, providing key data for biosafety assessment. However,… read more here.
Keywords: integration; dna; transgene integration; life seq ... See more keywords
The role of RBC antigen transgene integration sites on RBC biology in mice
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DOI: 10.1111/trf.17197
Abstract: Transgenic mice expressing RBC specific antigens are widely used in mechanistic studies of RBC alloimmunization. Existing RBC donor strains have random transgene integration, potentially disrupting host elements that can confound biological interpretation. read more here.
Keywords: rbc antigen; transgene integration; antigen transgene; role rbc ... See more keywords