CAMIO: a transgenic CRISPR pipeline to create diverse targeted genome deletions in Drosophila
Sign Up to like & getrecommendations! Published in 2020 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkaa177
Abstract: Abstract The genome is the blueprint for an organism. Interrogating the genome, especially locating critical cis-regulatory elements, requires deletion analysis. This is conventionally performed using synthetic constructs, making it cumbersome and non-physiological. Thus, we created… read more here.
Keywords: pipeline create; crispr pipeline; transgenic crispr; camio ... See more keywords
Non-Transgenic CRISPR-Mediated Knockout of Entire Ergot Alkaloid Gene Clusters in Slow-Growing Asexual Polyploid Fungi
Sign Up to like & getrecommendations! Published in 2021 at "Toxins"
DOI: 10.3390/toxins13020153
Abstract: The Epichloë species of fungi include seed-borne symbionts (endophytes) of cool-season grasses that enhance plant fitness, although some also produce alkaloids that are toxic to livestock. Selected or mutated toxin-free endophytes can be introduced into… read more here.
Keywords: crispr; transgenic crispr; crispr mediated; non transgenic ... See more keywords