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Published in 2020 at "Advanced Science"
DOI: 10.1002/advs.202000871
Abstract: Abstract The Legionella pneumophila effector MavC is a transglutaminase that carries out atypical ubiquitination of the host ubiquitin (Ub)‐conjugation enzyme UBE2N by catalyzing the formation of an isopeptide bond between Gln40Ub and Lys92UBE2N, which leads…
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Keywords:
mavc;
transglutaminase;
molecular basis;
basis ubiquitination ... See more keywords
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Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7398-9_18
Abstract: Transglutaminase (TGase:E.C. 2.3.2.13) catalyzes the acyl-transfer reaction between one or two primary amino groups of polyamines and protein-bound Gln residues giving rise to post-translational modifications. One increasing the positive charge on a proteins surface and…
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Keywords:
methodology;
transglutaminase;
tgase;
determination transglutaminase ... See more keywords
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Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9546-2_9
Abstract: Microbial transglutaminase (mTG), a protein-glutamine γ-glutamyltransferase from Streptomyces mobaraensis, is an enzyme capable of forming isopeptide bonds between the nearly inert (from the chemical point of view) γ-carboxamides present in the side chain of glutamine…
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Keywords:
transglutaminase;
mtg;
tailoring activity;
activity selectivity ... See more keywords
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Published in 2018 at "Amino Acids"
DOI: 10.1007/s00726-018-2635-2
Abstract: Previously we have demonstrated transglutaminase 2 (TGase 2) inhibition abrogated renal cell carcinoma (RCC) using GK921 (3-(phenylethynyl)-2-(2-(pyridin-2-yl)ethoxy)pyrido[3,2-b]pyrazine), although the mechanism of TGase 2 inhibition remains unsolved. Recently, we found that the increase of TGase 2…
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Keywords:
transglutaminase;
tgase;
inhibition;
allosteric inhibition ... See more keywords
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Published in 2017 at "Journal of Biosciences"
DOI: 10.1007/s12038-017-9707-4
Abstract: Abstract Microbial transglutaminase (MTG) gene (mtg) from Streptomyces hygroscopicus H197 strain was cloned by PCR and mutated by deleting a specific 84 bp fragment using overlapping extension PCR. The mutant MTG and the wild MTG genes…
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Keywords:
streptomyces hygroscopicus;
transglutaminase;
hygroscopicus h197;
mtg ... See more keywords
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Published in 2019 at "Bioresource technology"
DOI: 10.1016/j.biortech.2019.121391
Abstract: This work studied the production of Transglutaminase (TGase) using wheat bran as carbon source. The medium components and culture conditions were optimized by statistical Box-Behnken response surface methodology. The release of active Transglutaminase was enhanced…
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Keywords:
production;
transglutaminase;
utilization agro;
production transglutaminase ... See more keywords
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Published in 2021 at "Bioresource technology"
DOI: 10.1016/j.biortech.2021.125627
Abstract: Transglutaminase forms isopeptide bonds in proteins which are helpful in various industrial applications. However, low productivity and high cost are the major bottlenecks for industrial Transglutaminase production. The present study describes the regulatory mechanism of…
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Keywords:
production;
transglutaminase;
key regulators;
effect key ... See more keywords
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Published in 2020 at "Food chemistry"
DOI: 10.1016/j.foodchem.2020.127903
Abstract: Microbial transglutaminase (mTG) catalyses the formation of protein crosslinks, deamidating glutamine in a side-reaction. Gluten deamidation by human tissue transglutaminase is critical to activate celiac disease pathogenesis making the addition of mTG to wheat-based products…
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Keywords:
protein;
deamidation state;
transglutaminase;
mtg ... See more keywords
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Published in 2022 at "Food chemistry"
DOI: 10.1016/j.foodchem.2022.132210
Abstract: A novel mixture of glycopeptides was prepared from corn glutelin and glucosamine (GlcN). The functional properties and antioxidative activities of this mixture were investigated. Corn glutelin was limited hydrolyzed by Alcalase, and then its hydrolysates…
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Keywords:
conjugation glutelin;
transglutaminase;
glutelin hydrolysates;
functional properties ... See more keywords
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Published in 2017 at "Journal of Food Engineering"
DOI: 10.1016/j.jfoodeng.2016.10.020
Abstract: Semi-hard cheese samples made from pasteurized 3.5% and 5% fat cow’s milk were partly enzyme treated with microbial transglutaminase (mTG). Cheese colour and yield was greatly affected by mTG at both fat levels. The cross-linking…
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Keywords:
hyperspectral imaging;
transglutaminase;
effect;
semi hard ... See more keywords
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Published in 2017 at "Bioconjugate chemistry"
DOI: 10.1021/acs.bioconjchem.7b00509
Abstract: General approaches for controlled protein modification are increasingly sought-after in the arena of chemical biology. Here, using bioorthogonal reactions, we present combinatorial chemoenzymatic strategies to effectuate protein labeling. A total of three metal-free conjugations were…
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Keywords:
metal free;
transglutaminase;
chemistry;
one pot ... See more keywords