Synthesis of Adenine Nucleosides by Transglycosylation using Two Sequential Nucleoside Phosphorylase‐Based Bioreactors with On‐Line Reaction Monitoring by using HPLC
Sign Up to like & getrecommendations! Published in 2017 at "ChemCatChem"
DOI: 10.1002/cctc.201701222
Abstract: Uridine phosphorylase from Clostridium perfringens (CpUP, EC 2.4.2.3) was immobilized covalently in an aminopropylsilica monolithic column (25 mm×4.6 mm) upon functionalization with glutaraldehyde. Imino bonds that result from the reaction between the enzyme and the support were… read more here.
Keywords: line reaction; transglycosylation; phosphorylase; synthesis ... See more keywords
A novel acid‐tolerant β‐xylanase from Scytalidium candidum 3C for the synthesis of o‐nitrophenyl xylooligosaccharides
Sign Up to like & getrecommendations! Published in 2020 at "Journal of Basic Microbiology"
DOI: 10.1002/jobm.202000303
Abstract: Endo‐β‐xylanases are hemicellulases involved in the conversion of xylans in plant biomass. Here, we report a novel acidophilic β‐xylanase (ScXynA) with high transglycosylation abilities that was isolated from the filamentous fungus Scytalidium candidum 3C. ScXynA… read more here.
Keywords: scytalidium candidum; transglycosylation; nitrophenyl xylooligosaccharides; xylanase ... See more keywords
Synthesis of Chitin Oligosaccharides Using Dried Stenotrophomonas maltophilia Cells Containing a Transglycosylation Reaction-Catalyzing β-N-Acetylhexosaminidase as a Whole-Cell Catalyst
Sign Up to like & getrecommendations! Published in 2017 at "Applied Biochemistry and Biotechnology"
DOI: 10.1007/s12010-017-2585-2
Abstract: Bacterial strain NYT501, which we previously isolated from soil, was identified as Stenotrophomonas maltophilia, and it was confirmed that this strain produces an intracellular β-N-acetylhexosaminidase exhibiting transglycosylation activity. Several properties of this enzyme were characterized… read more here.
Keywords: whole cell; chitin oligosaccharides; transglycosylation; stenotrophomonas maltophilia ... See more keywords
Transglycosylation abilities of β-d-galactosidases from GH family 2
Sign Up to like & getrecommendations! Published in 2021 at "3 Biotech"
DOI: 10.1007/s13205-021-02715-w
Abstract: The ability to predict the transglycosylation activity of glycosidases by in silico analysis was investigated. The transglycosylation abilities of 7 different β-d-galactosidases from GH family 2 were tested experimentally using 7 different acceptors and p-nitrophenyl-β-d-galactopyranoside… read more here.
Keywords: abilities galactosidases; transglycosylation; galactosidases family; transglycosylation abilities ... See more keywords
Reaction specificity of keratanase II in the transglycosylation using the sugar oxazolines having keratan sulfate repeating units.
Sign Up to like & getrecommendations! Published in 2018 at "Carbohydrate research"
DOI: 10.1016/j.carres.2017.12.003
Abstract: The reaction specificity of the transglycosylation catalyzed by keratanase II from Bacillus circulans KsT202 (KSase II) was studied by using the oxazoline derivatives having keratan sulfate repeating units. The addition of 10% organic cosolvent reduced… read more here.
Keywords: reaction specificity; keratan sulfate; repeating units; transglycosylation ... See more keywords
GH13 Glycogen branching enzymes can adapt the substrate chain length towards their preferences via α-1,4-transglycosylation.
Sign Up to like & getrecommendations! Published in 2021 at "Enzyme and microbial technology"
DOI: 10.1016/j.enzmictec.2021.109882
Abstract: Glycogen branching enzymes (GBEs; 1,4-α-glucan branching enzyme; E.C. 2.4.1.18) have so far been described to be capable of both α-1,6-transglycosylation (branching) and α-1,4-hydrolytic activity. The aim of the present study was to elucidate the mode… read more here.
Keywords: chain length; transglycosylation; glycogen branching; activity ... See more keywords
GH36 α-galactosidase from Lactobacillus plantarum WCFS1 synthesize Gal-α-1,6 linked prebiotic α-galactooligosaccharide by transglycosylation.
Sign Up to like & getrecommendations! Published in 2019 at "International journal of biological macromolecules"
DOI: 10.1016/j.ijbiomac.2019.12.032
Abstract: α-Galactosidases are potent industrial glycoside hydrolases which are relatively less explored for their transglycosylation potential, especially from Lactobacillus genera. A GH36 α-galactosidase from Lactobacillus plantarum WCFS1 was cloned and over expressed in Hi-control Escherichia coli… read more here.
Keywords: plantarum wcfs1; transglycosylation; gh36 galactosidase;
Structural insights into β-glucosidase transglycosylation based on biochemical, structural and computational analysis of two GH1 enzymes from Trichoderma harzianum.
Sign Up to like & getrecommendations! Published in 2018 at "New biotechnology"
DOI: 10.1016/j.nbt.2017.08.012
Abstract: β-glucosidases are glycoside hydrolases able to cleave small and soluble substrates, thus producing monosaccharides. These enzymes are distributed among families GH1, GH2, GH3, GH5, GH9, GH30 and GH116, with GH1 and GH3 being the most… read more here.
Keywords: analysis; gh1; structural insights; transglycosylation ... See more keywords
Probing the determinants of the transglycosylation/hydrolysis partition in a retaining α-l-arabinofuranosidase.
Sign Up to like & getrecommendations! Published in 2021 at "New biotechnology"
DOI: 10.1016/j.nbt.2021.01.008
Abstract: The use of retaining glycoside hydrolases as synthetic tools for glycochemistry is highly topical and the focus of considerable research. However, due to the incomplete identification of the molecular determinants of the transglycosylation/hydrolysis partition (t/h),… read more here.
Keywords: hydrolysis partition; transglycosylation; hydrolysis; transglycosylation hydrolysis ... See more keywords
Substrate specificity and transglycosylation capacity of α-L-fucosidases across GH29 assessed by bioinformatics-assisted selection of functional diversity.
Sign Up to like & getrecommendations! Published in 2023 at "Glycobiology"
DOI: 10.1093/glycob/cwad029
Abstract: Glycoside hydrolase family 29 (GH29) encompasses α-L-fucosidases, i.e. enzymes which catalyze hydrolytic release of fucose from fucosylated glycans, including N- and O-linked glycans on proteins, and these α-L-fucosidases clearly play important roles in biology. GH29… read more here.
Keywords: gh29; cupp; specificity transglycosylation; transglycosylation ... See more keywords
Characteristics of an β-N-Acetylhexosaminidase from Bacillus sp. CH11, Including its Transglycosylation Activity.
Sign Up to like & getrecommendations! Published in 2018 at "Journal of food science"
DOI: 10.1111/1750-3841.14125
Abstract: β-N-Acetylhexosaminidase was identified from Bacillus sp. CH11 and found to have relatively high transferring activity. In this study, its enzymatic properties and transglycosylation activity including its acceptor specificity were investigated. Its molecular weight was estimated… read more here.
Keywords: transglycosylation activity; transglycosylation; acetylglucosaminyl xylitol; bacillus ch11 ... See more keywords