Cloning and characterization of a novel amylopullulanase from Bacillus megaterium Y103 with transglycosylation activity
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DOI: 10.1007/s10529-020-02891-4
Abstract: To obtain a novel pullulanase with synthetic ability from a microorganism and characterize its substrates specificity. A novel pullulanase, PulY103A, from Bacillus megaterium Y103 was purified, characterized and expressed in Escherichia coli. PulY103A contained the… read more here.
Keywords: transglycosylation activity; megaterium y103; activity; bacillus megaterium ... See more keywords
Key Residues Affecting Transglycosylation Activity in Family 18 Chitinases: Insights into Donor and Acceptor Subsites.
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DOI: 10.1021/acs.biochem.8b00381
Abstract: Understanding features that determine transglycosylation (TG) activity in glycoside hydrolases is important because it would allow the construction of enzymes that can catalyze controlled synthesis of oligosaccharides. To increase TG activity in two family 18… read more here.
Keywords: family chitinases; transglycosylation activity; activity; donor acceptor ... See more keywords
Characteristics of an β-N-Acetylhexosaminidase from Bacillus sp. CH11, Including its Transglycosylation Activity.
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DOI: 10.1111/1750-3841.14125
Abstract: β-N-Acetylhexosaminidase was identified from Bacillus sp. CH11 and found to have relatively high transferring activity. In this study, its enzymatic properties and transglycosylation activity including its acceptor specificity were investigated. Its molecular weight was estimated… read more here.
Keywords: transglycosylation activity; transglycosylation; acetylglucosaminyl xylitol; bacillus ch11 ... See more keywords
Characterization of a β-N-acetylhexosaminidase with transglycosylation activity from Metarhizium sp. A34.
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DOI: 10.1111/1750-3841.16113
Abstract: In applications of chitin, one of the most abundant resources on earth, human milk oligosaccharides with many health functions were synthesized by transglycosylation of β-N-acetylhexosaminidase. Synthesis of new transfer products can be expected by other… read more here.
Keywords: metarhizium a34; transglycosylation; transglycosylation activity; acetylhexosaminidase ... See more keywords
Transglycosylation Activity of Engineered Bifidobacterium Lacto-N-Biosidase Mutants at Donor Subsites for Lacto-N-Tetraose Synthesis
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DOI: 10.3390/ijms22063230
Abstract: The health benefits of human milk oligosaccharides (HMOs) make them attractive targets as supplements for infant formula milks. However, HMO synthesis is still challenging and only two HMOs have been marketed. Engineering glycoside hydrolases into… read more here.
Keywords: lacto biosidase; transglycosylation activity; lacto tetraose; activity ... See more keywords