Ready to use stocks of Agrobacterium tumefaciens can simplify process development for the production of recombinant proteins by transient expression in plants.
Sign Up to like & getrecommendations! Published in 2019 at "Biotechnology journal"
DOI: 10.1002/biot.201900113
Abstract: The transient expression of recombinant proteins in plant tissues infiltrated with Agrobacterium tumefaciens is an established basic research method that is also widely used for the development and manufacture of plant-made pharmaceuticals (PMPs). However, large-scale… read more here.
Keywords: transient expression; production; ready use; process ... See more keywords
Efficient base editing in tomato using a highly expressed transient system.
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DOI: 10.1007/s00299-021-02662-z
Abstract: KEY MESSAGE Base editing in tomatoes was achieved by transient expression. The Solanaceae plants, particularly the tomato (Solanum lycopersicum), is of huge economic value worldwide. The tomato is a unique model plant for studying the… read more here.
Keywords: base editing; transient expression; base; system ... See more keywords
Synthetic cold-inducible promoter enhances recombinant protein accumulation during Agrobacterium-mediated transient expression in Nicotiana excelsior at chilling temperatures
Sign Up to like & getrecommendations! Published in 2017 at "Biotechnology Letters"
DOI: 10.1007/s10529-017-2336-z
Abstract: ObjectivesTo exploit cold-inducible biochemical processes beneficial for foreign mRNA transcription, translation and storage, as well as protein product stability, during Agrobacterium-mediated transient expression.ResultsThe efficiency of three different 5′-regulatory sequences to achieve transient expression of the… read more here.
Keywords: transient expression; promoter; expression; cold inducible ... See more keywords
The transient expression of CHIKV VLP in large stirred tank bioreactors
Sign Up to like & getrecommendations! Published in 2019 at "Cytotechnology"
DOI: 10.1007/s10616-019-00346-x
Abstract: Transient gene expression (TGE) bioprocesses have been difficult to scale up in large stirred tank bioreactors with volumes of more than 1.5 L. Low production levels are often observed, but the causes have not been… read more here.
Keywords: stirred tank; transient expression; tank bioreactors; large stirred ... See more keywords
Optimization of Agrobacterium-mediated transient expression of heterologous genes in spinach
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DOI: 10.1007/s11816-017-0457-4
Abstract: The Agrobacterium-mediated transient assay is a relatively rapid technique and a promising approach for assessing the expression of a gene of interest. Despite the successful application of this transient expression system in several plant species,… read more here.
Keywords: transient expression; infiltration; agrobacterium mediated; expression ... See more keywords
Transient expression of anti-VEFGR2 nanobody in Nicotiana tabacum and N. benthamiana
Sign Up to like & getrecommendations! Published in 2018 at "3 Biotech"
DOI: 10.1007/s13205-018-1500-z
Abstract: In human, the interaction between vascular endothelial growth factor (VEGF) and its receptor (VEGFR2) is critical for tumor angiogenesis. This is a vital process for cancer tumor growth and metastasis. Blocking VEGF/VEGFR2 conjugation by antibodies… read more here.
Keywords: transient expression; anti vefgr2; tobacco plants; nanobody ... See more keywords
A protocol for functional study of genes in Brassica juncea by Agrobacterium-mediated transient expression: applicability in other Brassicaceae
Sign Up to like & getrecommendations! Published in 2019 at "Journal of Plant Biochemistry and Biotechnology"
DOI: 10.1007/s13562-019-00543-x
Abstract: Agrobacterium-mediated transient expression in plant organs is a quick and reliable method for studying gene functions. Due to the significance of transient transformation, substantial efforts have been dedicated to developing such protocols in various plants… read more here.
Keywords: transient expression; agrobacterium mediated; brassica juncea; mediated transient ... See more keywords
Transient expression of inactive RB in mesenchymal stem cells impairs their adipogenic potential and is associated with hypermethylation of the PPARγ2 promoter
Sign Up to like & getrecommendations! Published in 2020 at "Genes and Diseases"
DOI: 10.1016/j.gendis.2020.11.001
Abstract: Abstract The retinoblastoma gene product (pRb) is a chromatin-associated protein that can either suppress or promote activity of key regulators of tissue-specific differentiation. We found that twelve weeks after transfection of the exogenous active (ΔB/X… read more here.
Keywords: ppar; transient expression; expression; stem cells ... See more keywords
Transient expression of etanercept therapeutic protein in tobacco (Nicotiana tabacum L.).
Sign Up to like & getrecommendations! Published in 2019 at "International journal of biological macromolecules"
DOI: 10.1016/j.ijbiomac.2019.02.153
Abstract: Etanercept is a recombinant fusion protein of TNFR2 with the Fc portion of human IgG1. Etanercept, an anti-TNF drug, treats autoimmune diseases and improves patients' health. The main goal of the present study was to… read more here.
Keywords: protein; tobacco; transient expression; expression etanercept ... See more keywords
Transient expression of UreB of Helicobacter pylori in spinach (Spinacia oleracea)
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DOI: 10.1016/j.scienta.2018.12.018
Abstract: Abstract Helicobacter pylori that exists in the human gastric mucosa has been known the main cause of chronic gastritis, peptic ulcers and stomach cancer. Vaccination against H. pylori has been considered as the most effective… read more here.
Keywords: expression; pylori; helicobacter pylori; spinacia oleracea ... See more keywords
Transient Production of Recombinant Pharmaceutical Proteins in Plants: Evolution and Perspectives.
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DOI: 10.2174/0929867324666170718114724
Abstract: During the last two decades, the production of pharmaceutical proteins in plants evolved from proof of concept to established technology adopted by several biotechnological companies. This progress is particularly based on intensive research starting stable… read more here.
Keywords: transient expression; pharmaceutical proteins; production; expression ... See more keywords