Transient gene expression using valproic acid in combination with co‐transfection of SV40 large T antigen and human p21CIP/p27KIP
Sign Up to like & getrecommendations! Published in 2019 at "Biotechnology Progress"
DOI: 10.1002/btpr.2786
Abstract: Transient gene expression (TGE) in HEK293 cells was optimized by Vink et al. by co‐expression of human cell cycle inhibitors p21CIP/p27KIP and Simian virus 40 large T antigen (SVLT). In this study, we investigated the… read more here.
Keywords: p21cip p27kip; cell; transient gene; expression ... See more keywords
Transient gene expression in western white pine using agroinfiltration
Sign Up to like & getrecommendations! Published in 2019 at "Journal of Forestry Research"
DOI: 10.1007/s11676-019-00938-5
Abstract: A method for transient gene expression was developed for western white pine (WWP, Pinus monticola Dougl. ex D.Don) using reporter gene uidA encoding β -glucuronidase (GUS). GUS was transiently expressed in cross sections of primary… read more here.
Keywords: transient gene; agroinfiltration; gene expression; expression ... See more keywords
Efficient production of antibody Fab fragment by transient gene expression in insect cells.
Sign Up to like & getrecommendations! Published in 2017 at "Journal of bioscience and bioengineering"
DOI: 10.1016/j.jbiosc.2017.03.007
Abstract: Transient gene expression allows a rapid production of diverse recombinant proteins in early-stage preclinical and clinical developments of biologics. Insect cells have proven to be an excellent platform for the production of functional recombinant proteins.… read more here.
Keywords: production; transient gene; insect cells; gene expression ... See more keywords
Enhancement of tomato resistance to Tuta absoluta using a new efficient mesoporous silica nanoparticle-mediated plant transient gene expression approach
Sign Up to like & getrecommendations! Published in 2019 at "Scientia Horticulturae"
DOI: 10.1016/j.scienta.2018.08.040
Abstract: Abstract Due to their biodegradability and biocompatibility, mesoporous silica nanoparticles (MSNs) are employed as DNA nanocarriers in the following novel transient gene expression approach in tomato. The synthesized MSNs were analyzed in size, shape and… read more here.
Keywords: expression; mesoporous silica; transient gene; approach ... See more keywords
Developing Transient Gene Therapies to Decrease the Stability of Thrombi for Coagulopathy and Thrombosis
Sign Up to like & getrecommendations! Published in 2020 at "Blood"
DOI: 10.1182/blood-2020-143427
Abstract: Thrombotic disorders are prevalent and dangerous. Thrombosis involves the formation of unwanted blood clots inside blood vessels, which can impair blood flow and lead to severe cardiovascular events, such as heart attack, stroke, and pulmonary… read more here.
Keywords: transient gene; blood; gene therapies; thrombosis ... See more keywords