Transient Transformation Using Particle Bombardment for Gene Expression Analysis.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-8778-8_5
Abstract: Transient transformation or transient expression results in rapid and fleeting gene expression. This approach is often used as a first-tier screening tool for evaluation of components that affect gene expression. Here, we describe the use… read more here.
Keywords: transient transformation; particle bombardment; expression; gene expression ... See more keywords
Vacuum infiltration enhances the Agrobacterium-mediated transient transformation for gene functional analysis in persimmon (Diospyros kaki Thunb.)
Sign Up to like & getrecommendations! Published in 2019 at "Scientia Horticulturae"
DOI: 10.1016/j.scienta.2019.03.002
Abstract: Abstract An Agrobacterium-mediated transient transformation system was established for gene functional analysis in persimmon (Diospyros kaki Thunb.) utilizing sonication followed by vacuum infiltration of seedlings in vitro. The optimal vacuum conditions for agroinfiltration of persimmon… read more here.
Keywords: gene functional; vacuum infiltration; functional analysis; gene ... See more keywords
Modified carbon dot‐mediated transient transformation for genomic and epigenomic studies in wheat
Sign Up to like & getrecommendations! Published in 2025 at "Plant Biotechnology Journal"
DOI: 10.1111/pbi.14573
Abstract: Genotype restriction poses a significant bottleneck to stable transformation in the vast majority of plant species, thereby severely impeding advancement in plant bioengineering, particularly for crops. Nanoparticles (NPs) can serve as effective carriers for the… read more here.
Keywords: carbon dot; transient transformation; studies wheat; mediated transient ... See more keywords
Agrobacterium-mediated transient transformation of Flaveria bidentis leaves: a novel method to examine the evolution of C4 photosynthesis
Sign Up to like & getrecommendations! Published in 2024 at "Plant Methods"
DOI: 10.1186/s13007-024-01306-z
Abstract: The genus Flaveria has been studied extensively as a model for the evolution of C4 photosynthesis. Thus far, molecular analyses in this genus have been limited due to a dearth of genomic information and the… read more here.
Keywords: evolution photosynthesis; transient transformation; agrobacterium mediated; mediated transient ... See more keywords
An efficient and broadly applicable method for transient transformation of plants using vertically aligned carbon nanofiber arrays
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Plant Science"
DOI: 10.3389/fpls.2022.1051340
Abstract: Transient transformation in plants is a useful process for evaluating gene function. However, there is a scarcity of minimally perturbing methods for gene delivery that can be used on multiple organs, plant species, and non-excised… read more here.
Keywords: aligned carbon; transient transformation; plant; transformation plants ... See more keywords
Oxicam-type nonsteroidal anti-inflammatory drugs enhance Agrobacterium-mediated transient transformation in plants.
Sign Up to like & getrecommendations! Published in 2022 at "Plant biotechnology"
DOI: 10.5511/plantbiotechnology.22.0312a
Abstract: Agrobacterium-mediated transformation is a key innovation for plant breeding, and routinely used in basic researches and applied biology. However, the transformation efficiency is often the limiting factor of this technique. In this study, we discovered… read more here.
Keywords: oxicam type; mediated transient; agrobacterium mediated; transient transformation ... See more keywords