Fluorescent Translocation Reporters for Sub-plasma Membrane cAMP Imaging.
Sign Up to like & getrecommendations! Published in 2022 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-2245-2_20
Abstract: A wide range of fluorescent sensors with different properties have been developed for imaging of cAMP signals in living cells and tissues. Most cAMP reporters have been designed to undergo changes in fluorescence resonance energy… read more here.
Keywords: camp; membrane; plasma membrane; sub plasma ... See more keywords
Live-cell measurements of kinase activity in single cells using translocation reporters.
Sign Up to like & getrecommendations! Published in 2018 at "Nature protocols"
DOI: 10.1038/nprot.2017.128
Abstract: Although kinases are important regulators of many cellular processes, measuring their activity in live cells remains challenging. We have developed kinase translocation reporters (KTRs), which enable multiplexed measurements of the dynamics of kinase activity at… read more here.
Keywords: translocation reporters; kinase activity; microscopy; activity single ... See more keywords