A Mos1 transposase in vivo assay to screen new HIV-1 integrase inhibitors
Sign Up to like & getrecommendations! Published in 2018 at "Genetica"
DOI: 10.1007/s10709-018-0007-1
Abstract: The integrase and transposase enzymes of retrovirus and transposons, respectively, share the catalytic DDE domain. In vitro assays showed that inhibitors of HIV-1 integrase generally inhibit the mariner Mos1 transposase. Using a Drosophila strain in… read more here.
Keywords: integrase; mos1 transposase; transposase vivo; transposase ... See more keywords
The large bat Helitron DNA transposase forms a compact monomeric assembly that buries and protects its covalently bound 5'-transposon end.
Sign Up to like & getrecommendations! Published in 2021 at "Molecular cell"
DOI: 10.1016/j.molcel.2021.07.028
Abstract: Helitrons are widespread eukaryotic DNA transposons that have significantly contributed to genome variability and evolution, in part because of their distinctive, replicative rolling-circle mechanism, which often mobilizes adjacent genes. Although most eukaryotic transposases form oligomers… read more here.
Keywords: dna; end; transposase; helitron ... See more keywords
Structural basis of seamless excision and specific targeting by piggyBac transposase
Sign Up to like & getrecommendations! Published in 2020 at "Nature Communications"
DOI: 10.1038/s41467-020-17128-1
Abstract: The piggyBac DNA transposon is used widely in genome engineering applications. Unlike other transposons, its excision site can be precisely repaired without leaving footprints and it integrates specifically at TTAA tetranucleotides. We present cryo-EM structures… read more here.
Keywords: structural basis; dna; transposase; excision ... See more keywords
Engineered Sleeping Beauty transposase redirects transposon integration away from genes
Sign Up to like & getrecommendations! Published in 2022 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkac092
Abstract: Abstract The Sleeping Beauty (SB) transposon system is a popular tool for genome engineering, but random integration into the genome carries a certain genotoxic risk in therapeutic applications. Here we investigate the role of amino… read more here.
Keywords: transposon integration; integration; amino acids; transposase ... See more keywords
Recurrent evolution of vertebrate transcription factors by transposase capture
Sign Up to like & getrecommendations! Published in 2021 at "Science"
DOI: 10.1126/science.abc6405
Abstract: A recipe for new genes Most lineages contain evolutionarily novel genes, but their origin is not always clear. Cosby et al. investigated the origin of families of lineage-specific vertebrate genes (see the Perspective by Wacholder… read more here.
Keywords: protein; transcription; dna; transposase ... See more keywords
Enhanced Biosafety of the Sleeping Beauty Transposon System by Using mRNA as Source of Transposase to Efficiently and Stably Transfect Retinal Pigment Epithelial Cells
Sign Up to like & getrecommendations! Published in 2023 at "Biomolecules"
DOI: 10.3390/biom13040658
Abstract: Neovascular age-related macular degeneration (nvAMD) is characterized by choroidal neovascularization (CNV), which leads to retinal pigment epithelial (RPE) cell and photoreceptor degeneration and blindness if untreated. Since blood vessel growth is mediated by endothelial cell… read more here.
Keywords: pigment epithelial; pigment; rpe; transposase ... See more keywords
Functional Characterization of the N-Terminal Disordered Region of the piggyBac Transposase
Sign Up to like & getrecommendations! Published in 2022 at "International Journal of Molecular Sciences"
DOI: 10.3390/ijms231810317
Abstract: The piggyBac DNA transposon is an active element initially isolated from the cabbage looper moth, but members of this superfamily are also present in most eukaryotic evolutionary lineages. The functionally important regions of the transposase… read more here.
Keywords: piggybac; transposase; terminal disordered; disordered region ... See more keywords