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   Articles with "transposase" as a keyword



A Mos1 transposase in vivo assay to screen new HIV-1 integrase inhibitors

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recommendations! Published in 2018 at "Genetica"

DOI: 10.1007/s10709-018-0007-1

Abstract: The integrase and transposase enzymes of retrovirus and transposons, respectively, share the catalytic DDE domain. In vitro assays showed that inhibitors of HIV-1 integrase generally inhibit the mariner Mos1 transposase. Using a Drosophila strain in… read more here.

Keywords: integrase; mos1 transposase; transposase vivo; transposase ... See more keywords

The large bat Helitron DNA transposase forms a compact monomeric assembly that buries and protects its covalently bound 5'-transposon end.

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recommendations! Published in 2021 at "Molecular cell"

DOI: 10.1016/j.molcel.2021.07.028

Abstract: Helitrons are widespread eukaryotic DNA transposons that have significantly contributed to genome variability and evolution, in part because of their distinctive, replicative rolling-circle mechanism, which often mobilizes adjacent genes. Although most eukaryotic transposases form oligomers… read more here.

Keywords: dna; end; transposase; helitron ... See more keywords

Structural basis of seamless excision and specific targeting by piggyBac transposase

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recommendations! Published in 2020 at "Nature Communications"

DOI: 10.1038/s41467-020-17128-1

Abstract: The piggyBac DNA transposon is used widely in genome engineering applications. Unlike other transposons, its excision site can be precisely repaired without leaving footprints and it integrates specifically at TTAA tetranucleotides. We present cryo-EM structures… read more here.

Keywords: structural basis; dna; transposase; excision ... See more keywords

Engineered Sleeping Beauty transposase redirects transposon integration away from genes

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recommendations! Published in 2022 at "Nucleic Acids Research"

DOI: 10.1093/nar/gkac092

Abstract: Abstract The Sleeping Beauty (SB) transposon system is a popular tool for genome engineering, but random integration into the genome carries a certain genotoxic risk in therapeutic applications. Here we investigate the role of amino… read more here.

Keywords: transposon integration; integration; amino acids; transposase ... See more keywords

Engineering of BZ transposase and transposon donor vector for enhanced efficiency and safety in gene delivery applications

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recommendations! Published in 2025 at "Nucleic Acids Research"

DOI: 10.1093/nar/gkaf935

Abstract: Abstract Transposons, as non-viral vectors, provide an efficient and secure method for stable gene delivery and have been successfully applied in human gene therapies. The engineering of transposase has significantly improved the efficiency of various… read more here.

Keywords: efficiency; transposase; transposon; safety ... See more keywords

Interfering Transposable Elements: IS Xoo 15 Transposase as a First‐in‐Class Antibacterial Target Against Xanthomonas oryzae pv. oryzae

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recommendations! Published in 2025 at "Molecular Plant Pathology"

DOI: 10.1111/mpp.70169

Abstract: Current challenges in controlling phytopathogenic bacteria lie in widespread chemical resistance, biosafety concerns, and the scarcity of novel biomacromolecule targets. While transposable elements have emerged as critical drivers of genetic variability and virulence in plant… read more here.

Keywords: oryzae oryzae; pxo 03433; oryzae; transposable elements ... See more keywords

Recurrent evolution of vertebrate transcription factors by transposase capture

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recommendations! Published in 2021 at "Science"

DOI: 10.1126/science.abc6405

Abstract: A recipe for new genes Most lineages contain evolutionarily novel genes, but their origin is not always clear. Cosby et al. investigated the origin of families of lineage-specific vertebrate genes (see the Perspective by Wacholder… read more here.

Keywords: protein; transcription; dna; transposase ... See more keywords

Enhanced Biosafety of the Sleeping Beauty Transposon System by Using mRNA as Source of Transposase to Efficiently and Stably Transfect Retinal Pigment Epithelial Cells

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recommendations! Published in 2023 at "Biomolecules"

DOI: 10.3390/biom13040658

Abstract: Neovascular age-related macular degeneration (nvAMD) is characterized by choroidal neovascularization (CNV), which leads to retinal pigment epithelial (RPE) cell and photoreceptor degeneration and blindness if untreated. Since blood vessel growth is mediated by endothelial cell… read more here.

Keywords: pigment epithelial; pigment; rpe; transposase ... See more keywords

Functional Characterization of the N-Terminal Disordered Region of the piggyBac Transposase

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recommendations! Published in 2022 at "International Journal of Molecular Sciences"

DOI: 10.3390/ijms231810317

Abstract: The piggyBac DNA transposon is an active element initially isolated from the cabbage looper moth, but members of this superfamily are also present in most eukaryotic evolutionary lineages. The functionally important regions of the transposase… read more here.

Keywords: piggybac; transposase; terminal disordered; disordered region ... See more keywords