Accelerating and de‐risking CMC development with transposon‐derived manufacturing cell lines
Sign Up to like & getrecommendations! Published in 2021 at "Biotechnology and Bioengineering"
DOI: 10.1002/bit.27742
Abstract: The development of highly productive, genetically stable manufacturing cell lines is on the critical path to IND filing for protein‐based biologic drugs. Here, we describe the Leap‐In Transposase® platform, a novel transposon‐based mammalian (e.g., Chinese… read more here.
Keywords: cell lines; manufacturing cell; transposon; cell ... See more keywords
Using a modified piggyBac transposon-combined Cre/loxP system to produce selectable reporter-free transgenic bovine mammary epithelial cells for somatic cell nuclear transfer.
Sign Up to like & getrecommendations! Published in 2023 at "Genesis"
DOI: 10.1002/dvg.23510
Abstract: Transposon systems are widely used for genetic engineering in various model organisms. PiggyBac (PB) has recently been confirmed to have highly efficient transposition in the mouse germ line and mammalian cell lines. In this study,… read more here.
Keywords: system; cell; reporter; bovine mammary ... See more keywords
Mutant Construction and Integration Vector-Mediated Genetic Complementation in Listeria monocytogenes.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-0982-8_14
Abstract: Genes that play a role in stress response mechanisms and other phenotypes of Listeria monocytogenes can be identified by construction and screening of mutant libraries. In this chapter, we describe the construction and screening of… read more here.
Keywords: construction; transposon; listeria monocytogenes; integration vector ... See more keywords
Application of In Vitro Transposon Mutagenesis to Erythromycin Strain Improvement in Saccharopolyspora erythraea.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-6472-7_17
Abstract: Transposon mutagenesis is an invaluable technique in molecular biology for the creation of random mutations that can be easily identified and mapped. However, in the field of microbial strain improvement, transposon mutagenesis has scarcely been… read more here.
Keywords: transposon mutagenesis; biology; transposon; strain improvement ... See more keywords
Preparation of Transposon Library and Tn-Seq Amplicon Library for Salmonella Typhimurium.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9570-7_1
Abstract: Transposon sequencing (Tn-seq) is a powerful tool for functional genomics of bacteria. Tn-seq combines transposon mutagenesis with next generation sequencing to assess genetic requirements at a genome-wide scale and identify essential and conditionally essential genes.… read more here.
Keywords: transposon; seq amplicon; preparation; amplicon library ... See more keywords
piRNA-independent transposon silencing by the Drosophila THO complex.
Sign Up to like & getrecommendations! Published in 2021 at "Developmental cell"
DOI: 10.1016/j.devcel.2021.08.021
Abstract: piRNAs guide Piwi/Panoramix-dependent H3K9me3 chromatin modification and transposon silencing during Drosophila germline development. The THO RNA export complex is composed of Hpr1, Tho2, and Thoc5-7. Null thoc7 mutations, which displace Thoc5 and Thoc6 from a… read more here.
Keywords: independent transposon; transposon silencing; pirna independent; transposon ... See more keywords
Structure analysis of transposons carrying the aac(6')-aph(2″) gene in Enterococcus faecalis isolated in Beijing, China, and comparison of their transfer efficiency.
Sign Up to like & getrecommendations! Published in 2018 at "International journal of antimicrobial agents"
DOI: 10.1016/j.ijantimicag.2018.08.025
Abstract: Transfer of aac(6')-aph(2″) transposons mediating high-level gentamicin resistance (HLGR) in Enterococcus faecalis is a serious problem in the clinic. However, factors affecting the transfer of aac(6')-aph(2″) have not yet been elucidated. The current study aimed… read more here.
Keywords: location; aac aph; transposon; transfer ... See more keywords
Massive parallel insertion site sequencing of an arrayed Sinorhizobium meliloti signature-tagged mini-Tn 5 transposon mutant library.
Sign Up to like & getrecommendations! Published in 2017 at "Journal of biotechnology"
DOI: 10.1016/j.jbiotec.2017.02.019
Abstract: Transposon mutagenesis in conjunction with identification of genomic transposon insertion sites is a powerful tool for gene function studies. We have implemented a protocol for parallel determination of transposon insertion sites by Illumina sequencing involving… read more here.
Keywords: mutant library; insertion; transposon; signature tagged ... See more keywords
Large Drosophila germline piRNA clusters are evolutionarily labile and dispensable for transposon regulation
Sign Up to like & getrecommendations! Published in 2021 at "Molecular Cell"
DOI: 10.1016/j.molcel.2021.07.011
Abstract: Summary PIWI proteins and their guiding Piwi-interacting small RNAs (piRNAs) are crucial for fertility and transposon defense in the animal germline. In most species, the majority of piRNAs are produced from distinct large genomic loci,… read more here.
Keywords: pirna clusters; evolutionarily labile; transposon; transposon regulation ... See more keywords
Transposon-associated lincosamide resistance lnu(C) gene identified in Brachyspira hyodysenteriae ST83.
Sign Up to like & getrecommendations! Published in 2018 at "Veterinary microbiology"
DOI: 10.1016/j.vetmic.2017.12.003
Abstract: Treatment of Swine Dysentery (SD) caused by Brachyspira hyodysenteriae (B. hyodysenteriae) is carried out using antimicrobials such as macrolides, lincosamides and pleuromutilins leading to the selection of resistant strains. Whole genome sequencing of a multidrug-resistant… read more here.
Keywords: lincosamide resistance; brachyspira hyodysenteriae; hyodysenteriae; resistance ... See more keywords
Transposon sequencing analysis of Bradyrhizobium diazoefficiens 110spc4
Sign Up to like & getrecommendations! Published in 2021 at "Scientific Reports"
DOI: 10.1038/s41598-021-92534-z
Abstract: Bradyrhizobium diazoefficiens USDA110 is one of the most effective nitrogen-fixing symbionts of soybeans. Here we carried out a large-scale transposon insertion sequencing (Tn-seq) analysis of strain Bd110spc4, which is derived from USDA110, with the goal… read more here.
Keywords: transposon sequencing; transposon; sequencing analysis; bradyrhizobium diazoefficiens ... See more keywords