A Method of Transposon Insertion Sequencing in Comprehensively Identifying Vibrio vulnificus Genes Required for Growth in Human Serum.
Sign Up to like & getrecommendations! Published in 2022 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1720-5_9
Abstract: One of the most powerful approaches to detect the loci that enable a pathogen to cause disease is the creation of a high-density transposon mutant library by transposon insertion sequencing (TIS) and the screening of… read more here.
Keywords: pathogen; vulnificus; transposon insertion; serum ... See more keywords
Identification of Cancer Genes Based on De Novo Transposon Insertion Site Analysis Using RNA and DNA Sequencing.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-8967-6_5
Abstract: Forward genetic insertional mutagenesis screens are used by many labs to identify candidate cancer genes. We and others have used the Sleeping Beauty DNA transposon to generate random mutations within the murine genome that cause… read more here.
Keywords: cancer genes; transposon insertion; dna sequencing; cancer ... See more keywords
Transposon insertion resulted in the silencing of Wx-B1n in Chinese wheat landraces
Sign Up to like & getrecommendations! Published in 2017 at "Theoretical and Applied Genetics"
DOI: 10.1007/s00122-017-2878-4
Abstract: Key messageA novelWx-B1allele was characterized; a transposon insertion resulted in the loss of its function, which is different from the previously reported gene silencing mechanisms at theWx-B1locus.AbstractThe waxy protein composition of 53 Chinese wheat landraces… read more here.
Keywords: transposon insertion; insertion resulted; insertion; chinese wheat ... See more keywords
Experimental and Analytical Approaches for Improving the Resolution of Randomly Barcoded Transposon Insertion Sequencing (RB-TnSeq) Studies
Sign Up to like & getrecommendations! Published in 2022 at "ACS Synthetic Biology"
DOI: 10.1021/acssynbio.2c00119
Abstract: Randomly barcoded transposon insertion sequencing (RB-TnSeq) is an efficient, multiplexed method to determine microbial gene function during growth under a selection condition of interest. This technique applies to growth, tolerance, and persistence studies in a… read more here.
Keywords: transposon insertion; sequencing tnseq; experimental analytical; randomly barcoded ... See more keywords
Rapid ordering of barcoded transposon insertion libraries of anaerobic bacteria.
Sign Up to like & getrecommendations! Published in 2021 at "Nature protocols"
DOI: 10.1038/s41596-021-00531-3
Abstract: Commensal bacteria from the human intestinal microbiota play important roles in health and disease. Research into the mechanisms by which these bacteria exert their effects is hampered by the complexity of the microbiota, the strict… read more here.
Keywords: insertion libraries; microbiota; transposon insertion; insertion ... See more keywords
Human transposon insertion profiling by sequencing (TIPseq) to map LINE-1 insertions in single cells
Sign Up to like & getrecommendations! Published in 2020 at "Philosophical Transactions of the Royal Society B: Biological Sciences"
DOI: 10.1098/rstb.2019.0335
Abstract: Long interspersed element-1 (LINE-1, L1) sequences, which comprise about 17% of human genome, are the product of one of the most active types of mobile DNAs in modern humans. LINE-1 insertion alleles can cause inherited… read more here.
Keywords: transposon insertion; line; insertion; insertion profiling ... See more keywords
Mapping Transposon Insertion Sites by Inverse Polymerase Chain Reaction and Sanger Sequencing.
Sign Up to like & getrecommendations! Published in 2023 at "Cold Spring Harbor protocols"
DOI: 10.1101/pdb.prot108197
Abstract: Inverse polymerase chain reaction (PCR) is a method designed to amplify a segment of DNA for which only a portion of the sequence is known. The method consists of circularizing the DNA fragment by self-ligation… read more here.
Keywords: transposon insertion; chain reaction; polymerase chain; inverse polymerase ... See more keywords
Transposon Insertion Sequencing in a Clinical Isolate of Legionella pneumophila Identifies Essential Genes and Determinants of Natural Transformation
Sign Up to like & getrecommendations! Published in 2020 at "Journal of Bacteriology"
DOI: 10.1128/jb.00548-20
Abstract: Legionella pneumophila is the etiologic agent of a severe form of nosocomial and community-acquired pneumonia in humans. The environmental life traits of L. pneumophila are essential to its ability to accidentally infect humans. ABSTRACT Legionella… read more here.
Keywords: legionella pneumophila; transposon insertion; pneumophila; essential genes ... See more keywords
Revealing Causes for False-Positive and False-Negative Calling of Gene Essentiality in Escherichia coli Using Transposon Insertion Sequencing
Sign Up to like & getrecommendations! Published in 2022 at "mSystems"
DOI: 10.1128/msystems.00896-22
Abstract: Transposon mutagenesis is an efficient way to explore gene essentiality of a bacterial genome. However, there was a discrepancy between the essential gene set determined by transposon mutagenesis and that determined using single-gene knockout strains.… read more here.
Keywords: gene essentiality; gene; transposon insertion; insertion ... See more keywords
Identification of genetic elements required for Listeria monocytogenes growth under limited nutrient conditions and virulence by a screening of transposon insertion library
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Microbiology"
DOI: 10.3389/fmicb.2022.1007657
Abstract: Listeria monocytogenes, the causative agent of listeriosis, displays a lifestyle ranging from saprophytes in the soil to pathogenic as a facultative intracellular parasite in host cells. In the current study, a random transposon (Tn) insertion… read more here.
Keywords: transposon insertion; growth; insertion library; virulence ... See more keywords
Application of transposon insertion site sequencing method in the exploration of gene function in microalgae
Sign Up to like & getrecommendations! Published in 2023 at "Frontiers in Microbiology"
DOI: 10.3389/fmicb.2023.1111794
Abstract: Microalgae are a large group of organisms that can produce various useful substances through photosynthesis. Microalgae need to be genetically modified at the molecular level to become “Chassis Cells” for food, medicine, energy, and environmental… read more here.
Keywords: function microalgae; function; transposon insertion; sequencing method ... See more keywords