Transposon mutagenesis in Mycobacterium kansasii links a small RNA gene to colony morphology and biofilm formation and identifies 9,885 intragenic insertions that do not compromise colony outgrowth
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DOI: 10.1002/mbo3.988
Abstract: Mycobacterium kansasii (Mk) is a resilient opportunistic human pathogen that causes tuberculosis‐like chronic pulmonary disease and mortality stemming from comorbidities and treatment failure. The standard treatment of Mk infections requires costly, long‐term, multidrug courses with… read more here.
Keywords: small rna; colony; transposon mutagenesis; mycobacterium kansasii ... See more keywords
Application of In Vitro Transposon Mutagenesis to Erythromycin Strain Improvement in Saccharopolyspora erythraea.
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DOI: 10.1007/978-1-4939-6472-7_17
Abstract: Transposon mutagenesis is an invaluable technique in molecular biology for the creation of random mutations that can be easily identified and mapped. However, in the field of microbial strain improvement, transposon mutagenesis has scarcely been… read more here.
Keywords: transposon mutagenesis; biology; transposon; strain improvement ... See more keywords
Transposon Mutagenesis of Bacteroides fragilis.
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DOI: 10.1007/978-1-4939-9570-7_10
Abstract: Bacteroides fragilis is Gram-negative obligatory anaerobe which usually resides in the gut of humans and animals. As an important member of the human gut microbiota it plays a vital role in digestion and absorption of… read more here.
Keywords: mutagenesis bacteroides; bacteroides fragilis; transposon mutagenesis; biology ... See more keywords
Implementation of Transposon Mutagenesis in Bifidobacterium.
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DOI: 10.1007/978-1-4939-9570-7_5
Abstract: Random transposon mutagenesis allows for relatively rapid, genome-wide surveys to detect genes involved in functional traits, by performing screens of mutant libraries. This approach has been widely applied to identify genes responsible for activities of… read more here.
Keywords: mutagenesis; implementation transposon; transposon mutagenesis; bifidobacterium ... See more keywords
Identification of Genes Required for Swarming Motility in Bacillus subtilis Using Transposon Mutagenesis and High-Throughput Sequencing (TnSeq)
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DOI: 10.1128/jb.00089-22
Abstract: In TnSeq, transposons are randomly inserted throughout the chromosome at a population level, but insertions that disrupt genes of essential function cause strains that carry them to fall out of the population and appear underrepresented… read more here.
Keywords: genes required; transposon mutagenesis; bacillus subtilis; swarming motility ... See more keywords
Transposon Mutagenesis Reveals RBMS3 Silencing as a Promoter of Malignant Progression of BRAFV600E-Driven Lung Tumorigenesis
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DOI: 10.1158/0008-5472.can-21-3214
Abstract: Loss of RBMS3 cooperates with BRAFV600E to induce lung tumorigenesis, providing a deeper understanding of the molecular mechanisms underlying mutant BRAF-driven lung cancer and potential strategies to more effectively target this disease. read more here.
Keywords: transposon mutagenesis; mutagenesis reveals; driven lung; lung tumorigenesis ... See more keywords
Efficient transposon mutagenesis mediated by an IPTG-controlled conditional suicide plasmid
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DOI: 10.1186/s12866-018-1319-0
Abstract: BackgroundTransposon mutagenesis is highly valuable for bacterial genetic and genomic studies. The transposons are usually delivered into host cells through conjugation or electroporation of a suicide plasmid. However, many bacterial species cannot be efficiently conjugated… read more here.
Keywords: mutagenesis; conditional suicide; transposon mutagenesis; transposon ... See more keywords