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Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1048-0_14
Abstract: Fungi comprise one of the most diverse groups of eukaryotes with many cryptic species that are difficult to identify. In this chapter, we detail a protocol for the molecular identification of the most industrially relevant…
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Keywords:
trichoderma reesei;
biology;
identification;
identification trichoderma ... See more keywords
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Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1048-0_18
Abstract: Transcriptomics is a powerful technique to study gene expression. The main purpose of transcriptome studies in the filamentous fungus Trichoderma reesei is the analysis of differentially expressed genes as a transcriptional response of the genome…
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Keywords:
transcriptomics trichoderma;
methodology;
biology;
trichoderma reesei ... See more keywords
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Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1048-0_19
Abstract: A method based on reversed phase high-performance liquid chromatography coupled with electrospray ionization high-resolution mass spectrometry (RP-HPLC-ESI-HRMS) for the comprehensive and reliable detection of secondary metabolites of Trichoderma reesei cultured in synthetic minimal liquid medium…
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Keywords:
reliable detection;
trichoderma reesei;
comprehensive reliable;
metabolites trichoderma ... See more keywords
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Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1048-0_2
Abstract: Trichoderma reesei's potential as a rapid and efficient biomass degrader was first recognized in the 1950s when it was isolated from Army textiles during World War II. The microbe secreted cellulases that were degrading cotton-based…
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Keywords:
trichoderma reesei;
industrial relevance;
biology;
reesei ... See more keywords
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Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1048-0_5
Abstract: In this chapter, we describe a routinely used strategy for targeted gene insertions in Trichoderma reesei using auxotrophic markers. Generally, targeted gene integrations are advantageous over random, ectopic integration, because the copy number and locus…
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Keywords:
trichoderma reesei;
auxotrophic markers;
gene insertions;
gene ... See more keywords
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Published in 2021 at "Applied microbiology and biotechnology"
DOI: 10.1007/s00253-021-11261-7
Abstract: Biorefineries are core facilities for implementing a sustainable circular bioeconomy. These facilities rely on microbial enzymes to hydrolyze lignocellulosic substrates into fermentable sugars. Fungal co-cultures mimic the process of natural biodegradation and have been shown…
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Keywords:
production;
enzyme activities;
trichoderma reesei;
lignocellulolytic enzymes ... See more keywords
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Published in 2020 at "Current Microbiology"
DOI: 10.1007/s00284-020-01955-y
Abstract: The secretome of Trichoderma reesei contains a mixture of cellulases, hemicellulases, amylases, proteases, and lipases that synergistically degrade plant biomass. Trichodermapepsin (TrAsP), the most prominent protease of T. reesei, affects the stability of cellulases. Similar…
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Keywords:
xyr1 are1;
xyr1;
trichoderma reesei;
expression ... See more keywords
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Published in 2019 at "Cellulose"
DOI: 10.1007/s10570-019-02848-5
Abstract: Hydrothermal pre-treatments decrease lignocellulose recalcitrance against enzymatic hydrolysis by removing the majority of the hemicellulose, thus increasing cellulase accessibility. However, a small amount of the hemicellulose may remain and become adsorbed to the cellulose, leading…
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Keywords:
cel7a;
hemicellulose binding;
trichoderma reesei;
effect ... See more keywords
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Published in 2021 at "Applied Biochemistry and Biotechnology"
DOI: 10.1007/s12010-021-03529-5
Abstract: Trichoderma orientalis (T. orientalis) EU7-22 has a complete cellulase system and shows a remarkable enzyme activity with high potential in the industry. Ace2 is an important transcriptional factor for cellulase and hemicellulase expression in Trichoderma…
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Keywords:
orientalis eu7;
trichoderma reesei;
expression;
cellulase ... See more keywords
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Published in 2017 at "Molecular Biotechnology"
DOI: 10.1007/s12033-017-0018-7
Abstract: Trichoderma reesei strain Rut-C30 was modified with enhanced beta-glycosidase (BGL) activity to balance the cellulase system and generated laccase (LAC) protein for lignin degradation. Initially, the binary plasmid p1300-w1 was constructed to express T. reesei…
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Keywords:
random insertion;
trichoderma reesei;
beta;
reesei ... See more keywords
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Published in 2018 at "3 Biotech"
DOI: 10.1007/s13205-018-1421-x
Abstract: Experimental investigations were carried out to develop economic production process of cellulase using coconut mesocarp as an inexpensive lignocellulosic inducer while replacing commercial cellulose. Cellulase production was initially investigated from commercial cellulose in different submerged…
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Keywords:
trichoderma reesei;
cellulase;
coconut mesocarp;
production ... See more keywords