Reengineering a tRNA methyltransferase to covalently capture new RNA substrates.
Sign Up to like & getrecommendations! Published in 2019 at "Journal of the American Chemical Society"
DOI: 10.1021/jacs.9b08529
Abstract: Covalent RNA modifications can alter the function and metabolism of RNA transcripts. Altering the RNA substrate specificities of the enzymes that install these modifications can provide powerful tools to study and manipulate RNA. To develop… read more here.
Keywords: rna substrates; rna; trna methyltransferase; trma ... See more keywords
tRNA elbow modifications affect the tRNA pseudouridine synthase TruB and the methyltransferase TrmA.
Sign Up to like & getrecommendations! Published in 2020 at "RNA"
DOI: 10.1261/rna.075473.120
Abstract: tRNAs constitute the most highly modified class of RNA. Every tRNA contains a unique set of modifications, and Ψ55, m5U54, and m7G46 are frequently found within the elbow of the tRNA structure. Despite the abundance… read more here.
Keywords: trub; trna elbow; unmodified trna; trma ... See more keywords