Methods for CRISPR/Cas9 Xenopus tropicalis Tissue-Specific Multiplex Genome Engineering.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-8784-9_3
Abstract: In this chapter, we convey a state-of-the art update to the 2014 Nakayama protocol for CRISPR/Cas9 genome engineering in Xenopus tropicalis (X. tropicalis). We discuss in depth, gRNA design software and rules, gRNA synthesis, and procedures… read more here.
Keywords: crispr cas9; methodology; tissue specific; tropicalis ... See more keywords
Electrospun essential oil-polycaprolactone nanofibers as antibiofilm surfaces against clinical Candida tropicalis isolates
Sign Up to like & getrecommendations! Published in 2019 at "Biotechnology Letters"
DOI: 10.1007/s10529-019-02660-y
Abstract: ObjectiveAs an approach to prevent biofilm infections caused by Candida tropicalis on various surfaces, determination of effect of biodegradable polycaprolactone nanofibers (PCLNFs) with different concentrations of two different essential oils were tested in this study.ResultsBoth… read more here.
Keywords: essential oil; oil pclnfs; pclnfs; red thyme ... See more keywords
The Activities of Adhesion and Biofilm Formation by Candida tropicalis Clinical Isolates Display Significant Correlation with Its Multilocus Sequence Typing
Sign Up to like & getrecommendations! Published in 2017 at "Mycopathologia"
DOI: 10.1007/s11046-017-0111-2
Abstract: Adhesion and biofilm formation, which can occur on abiotic and biotic surfaces, are key components in Candida pathogenicity. The aims of this study were to infer about the C. tropicalis clinical isolates ability to adhere… read more here.
Keywords: tropicalis; biofilm formation; adhesion; adhesion biofilm ... See more keywords
Murine model for the evaluation of candiduria caused by Candida tropicalis from biofilm.
Sign Up to like & getrecommendations! Published in 2018 at "Microbial pathogenesis"
DOI: 10.1016/j.micpath.2018.02.036
Abstract: To evaluate the pathophysiology of catheter-associated candiduria, the bladders of female mice were infected with Candida tropicalis. One group was implanted with a catheter fragment with preformed biofilm by cystotomy technique, while another group received,… read more here.
Keywords: catheter; tropicalis; model; biofilm ... See more keywords
In vitro interaction of Candida tropicalis biofilm formed on catheter with human cells.
Sign Up to like & getrecommendations! Published in 2018 at "Microbial pathogenesis"
DOI: 10.1016/j.micpath.2018.09.029
Abstract: Candida tropicalis has emerged as one of the major Candida non-C. albicans species, in terms of epidemiology and virulence. Despite its virulence, C. tropicalis pathogenic mechanism has yet not been fully defined. The current study… read more here.
Keywords: tropicalis biofilm; candida; tropicalis; biofilm ... See more keywords
Convergent and divergent roles of the glucose-responsive kinase SNF4 in Candida tropicalis
Sign Up to like & getrecommendations! Published in 2023 at "Virulence"
DOI: 10.1080/21505594.2023.2175914
Abstract: ABSTRACT The sucrose non-fermenting 1 (SNF1) complex is a heterotrimeric protein kinase complex that is an ortholog of the mammalian AMPK complex and is evolutionally conserved in most eukaryotes. This complex contains a catalytic subunit… read more here.
Keywords: candida tropicalis; divergent roles; convergent divergent; snf4 ... See more keywords
Candida tropicalis Isolates from Mexican Republic Exhibit High Susceptibility to Bleomycin and Variable Susceptibility to Hydrogen Peroxide.
Sign Up to like & getrecommendations! Published in 2018 at "Microbial drug resistance"
DOI: 10.1089/mdr.2017.0253
Abstract: Candida sp. are found as part of the commensal flora in humans but can cause invasive candidiasis in patients with severe underlying disease, especially cancer patients. These patients are frequently subjected to nonsurgical anticancer treatments… read more here.
Keywords: high susceptibility; susceptibility; susceptibility hydrogen; hydrogen peroxide ... See more keywords
Pan-azole-resistant Candida tropicalis carrying homozygous erg11 mutations at position K143R: a new emerging superbug?
Sign Up to like & getrecommendations! Published in 2017 at "Journal of Antimicrobial Chemotherapy"
DOI: 10.1093/jac/dkw558
Abstract: Objectives Candidaemia is a public health problem mainly in hospitalized individuals worldwide. In Brazil, Candida albicans is the most prevalent species that causes candidaemia, followed by Candida tropicalis and Candida parapsilosis . Few data on… read more here.
Keywords: pan azole; candida; tropicalis; resistance ... See more keywords
Antifungal susceptibility, genotyping, resistance mechanism, and clinical profile of Candida tropicalis blood isolates
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DOI: 10.1093/mmy/myz124
Abstract: Abstract Candida tropicalis is one of the major candidaemia agents, associated with the highest mortality rates among Candida species, and developing resistance to azoles. Little is known about the molecular mechanisms of azole resistance, genotypic… read more here.
Keywords: tropicalis; resistance; tropicalis blood; antifungal susceptibility ... See more keywords
Best Practices for Xenopus tropicalis Husbandry.
Sign Up to like & getrecommendations! Published in 2022 at "Cold Spring Harbor protocols"
DOI: 10.1101/pdb.top106252
Abstract: Xenopus tropicalis has been adopted by laboratories as a developmental genetic system because of its diploid genome and short generation time, contrasting with Xenopus laevis, which is allotetraploid and takes longer to reach sexual maturity.… read more here.
Keywords: tropicalis husbandry; best practices; xenopus tropicalis; practices xenopus ... See more keywords
Development of a gRNA Expression and Processing Platform for Efficient CRISPR-Cas9-Based Gene Editing and Gene Silencing in Candida tropicalis
Sign Up to like & getrecommendations! Published in 2022 at "Microbiology Spectrum"
DOI: 10.1128/spectrum.00059-22
Abstract: In the nonmodel yeast Candida tropicalis, a lack of available RNA polymerase type III (Pol III) promoters hindered the development of guide RNA (gRNA) expression platforms for the establishment of CRISPR-Cas-mediated genome editing and silencing… read more here.
Keywords: platform; crispr; trna grna; tropicalis ... See more keywords