Structural destabilization of tropomyosin induced by the cardiomyopathy‐linked mutation R21H
Sign Up to like & getrecommendations! Published in 2018 at "Protein Science"
DOI: 10.1002/pro.3341
Abstract: The missense mutation R21H in striated muscle tropomyosin is associated with hypertrophic cardiomyopathy, a genetic cardiac disease and a leading cause of sudden cardiac death in young people. Tropomyosin adopts conformation of a coiled coil… read more here.
Keywords: tm1a1 28zip; structural destabilization; tropomyosin; mutation r21h ... See more keywords
Allergenicity of tropomyosin of shrimp (Litopenaeus vannamei) and clam (Ruditapes philippinarum) is higher than that of fish (Larimichthys crocea) via in vitro and in vivo assessment
Sign Up to like & getrecommendations! Published in 2019 at "European Food Research and Technology"
DOI: 10.1007/s00217-019-03402-0
Abstract: Tropomyosin (TM), acting as heat-stable protein, has a high homology of amino acid sequences with different sensitizations. Fish belongs to vertebrate and its TM owns less allergenicity, whereas shrimp and clam belong to invertebrate and… read more here.
Keywords: clam; allergenicity; tropomyosin; shrimp ... See more keywords
The primary cause of muscle disfunction associated with substitutions E240K and R244G in tropomyosin is aberrant behavior of tropomyosin and response of actin and myosin during ATPase cycle.
Sign Up to like & getrecommendations! Published in 2018 at "Archives of biochemistry and biophysics"
DOI: 10.1016/j.abb.2018.03.002
Abstract: Using the polarized photometry technique we have studied the effects of two amino acid replacements, E240K and R244G, in tropomyosin (Tpm1.1) on the position of Tpm1.1 on troponin-free actin filaments and the spatial arrangement of… read more here.
Keywords: e240k r244g; actin; muscle; tropomyosin ... See more keywords
Temperature sensitive point mutations in fission yeast tropomyosin have long range effects on the stability and function of the actin-tropomyosin copolymer
Sign Up to like & getrecommendations! Published in 2018 at "Biochemical and Biophysical Research Communications"
DOI: 10.1016/j.bbrc.2017.10.109
Abstract: The actin cytoskeleton is modulated by regulatory actin-binding proteins which fine-tune the dynamic properties of the actin polymer to regulate function. One such actin-binding protein is tropomyosin (Tpm), a highly-conserved alpha-helical dimer which stabilises actin… read more here.
Keywords: point mutations; tropomyosin; temperature sensitive; function ... See more keywords
The reason for a high Ca2+-sensitivity associated with Arg91Gly substitution in TPM2 gene is the abnormal behavior and high flexibility of tropomyosin during the ATPase cycle.
Sign Up to like & getrecommendations! Published in 2017 at "Biochemical and biophysical research communications"
DOI: 10.1016/j.bbrc.2017.10.161
Abstract: Substitution of Arg for Gly residue in 91th position in β-tropomyosin caused by a point mutation in TPM2 gene is associated with distal arthrogryposis, characterized by a high Ca2+-sensitivity of myofilament and contracture syndrome. To… read more here.
Keywords: high ca2; tropomyosin; ca2 sensitivity; atpase cycle ... See more keywords
C-terminal troponin-I residues trap tropomyosin in the muscle thin filament blocked-state.
Sign Up to like & getrecommendations! Published in 2021 at "Biochemical and biophysical research communications"
DOI: 10.1016/j.bbrc.2021.03.010
Abstract: Tropomyosin and troponin regulate muscle contraction by participating in a macromolecular scale steric-mechanism to control myosin-crossbridge - actin interactions and consequently contraction. At low-Ca2+, the C-terminal 30% of troponin subunit-I (TnI) is proposed to trap… read more here.
Keywords: muscle; switch peptide; tropomyosin; low ca2 ... See more keywords
The Effect of Tropomyosin Mutations on Actin-Tropomyosin Binding: In Search of Lost Time.
Sign Up to like & getrecommendations! Published in 2019 at "Biophysical journal"
DOI: 10.1016/j.bpj.2019.05.009
Abstract: The initial binding of tropomyosin onto actin filaments and then its polymerization into continuous cables on the filament surface must be precisely tuned to overall thin-filament structure, function, and performance. Low-affinity interaction of tropomyosin with… read more here.
Keywords: effect tropomyosin; tropomyosin; tropomyosin binding; actin tropomyosin ... See more keywords
Co-polymers of Actin and Tropomyosin Account for a Major Fraction of the Human Actin Cytoskeleton
Sign Up to like & getrecommendations! Published in 2018 at "Current Biology"
DOI: 10.1016/j.cub.2018.05.053
Abstract: Tropomyosin proteins form stable coiled-coil dimers that polymerize along the α-helical groove of actin filaments [1]. The actin cytoskeleton consists of both co-polymers of actin and tropomyosin and polymers of tropomyosin-free actin [2]. The fundamental… read more here.
Keywords: actin cytoskeleton; actin; tropomyosin; actin tropomyosin ... See more keywords
cGMP interacts with tropomyosin and downregulates actin-tropomyosin-myosin complex interaction
Sign Up to like & getrecommendations! Published in 2018 at "Respiratory Research"
DOI: 10.1186/s12931-018-0903-z
Abstract: BackgroundThe nitric oxide-soluble guanylate cyclase-cyclic guanosine monophosphate (NO-sGC-cGMP) signaling pathway, plays a critical role in the pathogenesis of pulmonary arterial hypertension (PAH); however, its exact molecular mechanism remains undefined.MethodsBiotin-cGMP pull-down assay was performed to search… read more here.
Keywords: tropomyosin myosin; tropomyosin; cgmp; actin tropomyosin ... See more keywords
Parallel assembly of actin and tropomyosin, but not myosin II, during de novo actin filament formation in live mice
Sign Up to like & getrecommendations! Published in 2018 at "Journal of Cell Science"
DOI: 10.1242/jcs.212654
Abstract: ABSTRACT Many actin filaments in animal cells are co-polymers of actin and tropomyosin. In many cases, non-muscle myosin II associates with these co-polymers to establish a contractile network. However, the temporal relationship of these three… read more here.
Keywords: myosin; tropomyosin; actin tropomyosin; assembly actin ... See more keywords
mNG-tagged fusion proteins and nanobodies to visualize tropomyosins in yeast and mammalian cells
Sign Up to like & getrecommendations! Published in 2022 at "Journal of Cell Science"
DOI: 10.1242/jcs.260288
Abstract: ABSTRACT Tropomyosins are structurally conserved α-helical coiled-coil proteins that bind along the length of filamentous actin (F-actin) in fungi and animals. Tropomyosins play essential roles in the stability of actin filaments and in regulating myosin… read more here.
Keywords: mng tagged; fusion proteins; tropomyosin; proteins nanobodies ... See more keywords