Novel proteinase inhibitor from the hemolymph of soybean pest Anticarsia gemmatalis (lepidóptera: Noctuidae): Structural modeling and enzymatic kinetic.
Sign Up to like & getrecommendations! Published in 2022 at "Archives of insect biochemistry and physiology"
DOI: 10.1002/arch.21864
Abstract: New approaches are needed to reduce risks to the environment and natural enemies and to avoid or delay the onset of insecticide resistance. The use of insecticides based on proteinase inhibitors of hemolymph is an… read more here.
Keywords: trypsin; anticarsia gemmatalis; gemmatalis; inhibitor ... See more keywords
Flow cytometric detection of most proteins in the cell surface proteome is unaffected by trypsin treatment
Sign Up to like & getrecommendations! Published in 2018 at "Cytometry Part A"
DOI: 10.1002/cyto.a.23525
Abstract: Flow cytometry is often performed on adherent cells or solid tissues that have been released from their growth substrate or disaggregated by enzymatic digestion. Although detection of strongly expressed cell surface proteins following such procedures… read more here.
Keywords: detection; treatment; trypsin; trypsin treatment ... See more keywords
A substrate for a cell free in vitro assay system to screen drugs targeting trypsin like protease‐based cleavage of SARS‐CoV‐2 spike glycoprotein and viral entry
Sign Up to like & getrecommendations! Published in 2023 at "Journal of Medical Virology"
DOI: 10.1002/jmv.28796
Abstract: Host proteases trypsin and trypsin‐like proteases have been reported to facilitate the entry of coronavirus SARS‐CoV‐2 in its host cells. These protease enzymes cleave the viral surface glycoprotein, spike, leading to successful cell surface receptor… read more here.
Keywords: trypsin; cleavage; substrate; trypsin like ... See more keywords
On possible trypsin‐induced biases in peptides analysis with aerolysin nanopore
Sign Up to like & getrecommendations! Published in 2022 at "PROTEOMICS"
DOI: 10.1002/pmic.202100056
Abstract: Nanopore‐based single‐molecule analysis technique is a promising approach in the field of proteomics. In this Technical Brief, the interaction between the biological nanopore of Aerolysin (AeL) and peptides is investigated, focusing on potential biases depending… read more here.
Keywords: trypsin; aerolysin; analysis; activation protocol ... See more keywords
Isolation, purification and characterization of a pH tolerant and temperature stable proteinaceous protease inhibitor from marine Pseudomonas mendocina
Sign Up to like & getrecommendations! Published in 2017 at "Biotechnology Letters"
DOI: 10.1007/s10529-017-2424-0
Abstract: ObjectivesAn extracellular protease inhibitor (BTPI-301) of trypsin was purified and characterized from an isolate of Pseudomonas mendocina.ResultsBTPI-301was purified to homogeneity by (NH4)2SO4, precipitation, DEAE Sepharose and CNBr-activated Sepharose chromatography. Homogeneity was proved by native PAGE… read more here.
Keywords: protease inhibitor; btpi 301; inhibitor; trypsin ... See more keywords
Extraction of gelatin from salmon (Salmo salar) fish skin using trypsin-aided process: optimization by Plackett–Burman and response surface methodological approaches
Sign Up to like & getrecommendations! Published in 2017 at "Journal of Food Science and Technology"
DOI: 10.1007/s13197-017-2864-5
Abstract: Gelatin from salmon (Salmo salar) skin with high molecular weight protein chains (α-chains) was extracted using trypsin-aided process. Response surface methodology was used to optimise the extraction parameters. Yield, hydroxyproline content and protein electrophoretic profile… read more here.
Keywords: extraction; response surface; process; trypsin ... See more keywords
A new disruption observed in the white shrimp Penaeus vannamei caused by the absence of a trypsin allele
Sign Up to like & getrecommendations! Published in 2020 at "Aquaculture"
DOI: 10.1016/j.aquaculture.2020.735191
Abstract: Abstract In a previous study, the frequency of three different trypsin genotypes of Penaeus vannamei in aquaculture was described. Genotype CBA was the most abundant, followed by genotype CB, but genotype CA decreased its frequency… read more here.
Keywords: penaeus vannamei; absence; aquaculture; genotype ... See more keywords
Mesenchymal stem cell detachment with trace trypsin is superior to EDTA for in vitro chemotaxis and adhesion assays.
Sign Up to like & getrecommendations! Published in 2017 at "Biochemical and biophysical research communications"
DOI: 10.1016/j.bbrc.2017.01.171
Abstract: Trypsin is frequently used to dissociate mesenchymal stem cells (MSCs) for in vitro adhesion and chemotaxis assays. However, its potential impact on surface receptor degradation is poorly understood. The purpose of this study was to evaluate… read more here.
Keywords: edta; pbs edta; adhesion; trypsin ... See more keywords
Role of host trypsin-type serine proteases and influenza virus-cytokine-trypsin cycle in influenza viral pathogenesis. Pathogenesis-based therapeutic options.
Sign Up to like & getrecommendations! Published in 2019 at "Biochimie"
DOI: 10.1016/j.biochi.2019.09.006
Abstract: Influenza A virus (IAV) is one of the most common infectious pathogen and associated with significant morbidity and mortality. Although processing the IAV hemagglutinin (HA) envelope glycoprotein precursor is a pre-requisite for viral membrane fusion… read more here.
Keywords: cytokine; pathogenesis; cycle; trypsin ... See more keywords
Allostery in Coagulation Factor VIIa Revealed by Ensemble Refinement of Crystallographic Structures.
Sign Up to like & getrecommendations! Published in 2019 at "Biophysical journal"
DOI: 10.1016/j.bpj.2019.03.024
Abstract: A critical step in injury-induced initiation of blood coagulation is the formation of the complex between the trypsin-like protease coagulation factor VIIa (FVIIa) and its cofactor tissue factor (TF), which converts FVIIa from an intrinsically… read more here.
Keywords: coagulation; ensemble refinement; trypsin; crystallographic structures ... See more keywords
Protein-Protein Binding as a Two-Step Mechanism: Preselection of Encounter Poses during the Binding of BPTI and Trypsin
Sign Up to like & getrecommendations! Published in 2020 at "Biophysical Journal"
DOI: 10.1016/j.bpj.2020.06.032
Abstract: Biomolecular recognition between proteins follows complex mechanisms, the understanding of which can substantially advance drug discovery efforts. Here, we track each step of the binding process in atomistic detail with molecular dynamics simulations using trypsin… read more here.
Keywords: protein; step; encounter; process ... See more keywords