On-column trypsin digestion coupled with LC-MS/MS for quantification of apolipoproteins.
Sign Up to like & getrecommendations! Published in 2017 at "Journal of proteomics"
DOI: 10.1016/j.jprot.2016.09.011
Abstract: Apolipoproteins measured in plasma or serum are potential biomarkers for assessing metabolic irregularities that are associated with the development of cardiovascular disease (CVD). LC-MS/MS allows quantitative measurement of multiple apolipoproteins in the same sample run.… read more here.
Keywords: coupled quantification; trypsin digestion; digestion; application ... See more keywords
A High-Throughput Workflow for FFPE Tissue Proteomics.
Sign Up to like & getrecommendations! Published in 2023 at "Journal of the American Society for Mass Spectrometry"
DOI: 10.1021/jasms.3c00099
Abstract: Laser capture microdissection (LCM) has become an indispensable tool for mass spectrometry-based proteomic analysis of specific regions obtained from formalin-fixed paraffin-embedded (FFPE) tissue samples in both clinical and research settings. Low protein yields from LCM… read more here.
Keywords: ffpe tissue; sample processing; trypsin digestion; high throughput ... See more keywords
Revisiting the Effect of Trypsin Digestion Buffers on Artificial Deamidation.
Sign Up to like & getrecommendations! Published in 2025 at "Journal of the American Society for Mass Spectrometry"
DOI: 10.1021/jasms.4c00389
Abstract: Deamidation of asparagine and glutamine residues occurs spontaneously, is influenced by pH, temperature, and incubation time, and can be accelerated by adjacent amino acid residues. Incubation conditions used for proteolytic digestion in bottom-up proteomic studies… read more here.
Keywords: trypsin digestion; revisiting effect; artificial deamidation; digestion ... See more keywords