On-column trypsin digestion coupled with LC-MS/MS for quantification of apolipoproteins.
Sign Up to like & getrecommendations! Published in 2017 at "Journal of proteomics"
DOI: 10.1016/j.jprot.2016.09.011
Abstract: Apolipoproteins measured in plasma or serum are potential biomarkers for assessing metabolic irregularities that are associated with the development of cardiovascular disease (CVD). LC-MS/MS allows quantitative measurement of multiple apolipoproteins in the same sample run.… read more here.
Keywords: coupled quantification; trypsin digestion; digestion; application ... See more keywords
A High-Throughput Workflow for FFPE Tissue Proteomics.
Sign Up to like & getrecommendations! Published in 2023 at "Journal of the American Society for Mass Spectrometry"
DOI: 10.1021/jasms.3c00099
Abstract: Laser capture microdissection (LCM) has become an indispensable tool for mass spectrometry-based proteomic analysis of specific regions obtained from formalin-fixed paraffin-embedded (FFPE) tissue samples in both clinical and research settings. Low protein yields from LCM… read more here.
Keywords: ffpe tissue; sample processing; trypsin digestion; high throughput ... See more keywords