A substrate for a cell free in vitro assay system to screen drugs targeting trypsin like protease‐based cleavage of SARS‐CoV‐2 spike glycoprotein and viral entry
Sign Up to like & getrecommendations! Published in 2023 at "Journal of Medical Virology"
DOI: 10.1002/jmv.28796
Abstract: Host proteases trypsin and trypsin‐like proteases have been reported to facilitate the entry of coronavirus SARS‐CoV‐2 in its host cells. These protease enzymes cleave the viral surface glycoprotein, spike, leading to successful cell surface receptor… read more here.
Keywords: trypsin; cleavage; substrate; trypsin like ... See more keywords
Identification and annotation of bovine granzyme genes reveals a novel granzyme encoded within the trypsin-like locus
Sign Up to like & getrecommendations! Published in 2018 at "Immunogenetics"
DOI: 10.1007/s00251-018-1062-6
Abstract: Granzymes are a family of serine proteases found in the lytic granules of cytotoxic T lymphocytes and natural killer (NK) cells, which are involved in killing of susceptible target cells. Most information on granzymes and… read more here.
Keywords: trypsin like; granzyme genes; annotation bovine; granzyme ... See more keywords
Human airway trypsin-like protease enhances interleukin-8 synthesis in bronchial epithelial cells by activating protease-activated receptor 2.
Sign Up to like & getrecommendations! Published in 2019 at "Archives of biochemistry and biophysics"
DOI: 10.1016/j.abb.2019.01.019
Abstract: Human airway trypsin-like protease (HAT) localizes at human bronchial epithelial cells (HBECs). HAT enhanced release of interleukin-8 (IL-8) from HBECs at 10-100 mU/mL and the enhanced release was almost completely abolished by 50 μM leupeptin, a… read more here.
Keywords: par2; trypsin like; epithelial cells; protease ... See more keywords
A trypsin-like serine protease domain of masquerade gene in crayfish Procambarus clarkii could activate prophenoloxidase and inhibit bacterial growth.
Sign Up to like & getrecommendations! Published in 2020 at "Developmental and comparative immunology"
DOI: 10.1016/j.dci.2020.103980
Abstract: Masquerade (Mas) is a secreted trypsin-like serine protease (SPs) and involved in immune response in some arthropods. However, according to previous studies, Mas presents different functional activities. In the present study, the functional mechanisms of… read more here.
Keywords: trypsin like; serine protease; expression; pcmas ... See more keywords
Coevolved Positions Represent Key Functional Properties in the Trypsin-Like Serine Proteases Protein Family
Sign Up to like & getrecommendations! Published in 2020 at "Journal of chemical information and modeling"
DOI: 10.1021/acs.jcim.9b00903
Abstract: Trypsin-like Serine Proteases are a group of homologous enzymes which exert multiple roles in both vertebrate and invertebrate organisms. Key properties of these enzymes include their activation from an inactive zymogen form to their active… read more here.
Keywords: trypsin like; like serine; serine proteases; protein family ... See more keywords
Evaluation of the ability of the trypsin-like peptidase activity assay to detect severe periodontitis
Sign Up to like & getrecommendations! Published in 2021 at "PLoS ONE"
DOI: 10.1371/journal.pone.0256538
Abstract: Objectives N-benzoyl-DL-arginine peptidase (trypsin-like peptidase) is specifically produced by certain strains of periodontitis-associated bacteria. We aimed to examine the effectiveness of an objectively quantified trypsin-like peptidase activity assay (TLP-AA) for detecting severe periodontitis. Methods The… read more here.
Keywords: trypsin like; tlp results; severe periodontitis; like peptidase ... See more keywords
Chemically diverse activity-based probes with unexpected inhibitory mechanisms targeting trypsin-like serine proteases
Sign Up to like & getrecommendations! Published in 2023 at "Frontiers in Chemistry"
DOI: 10.3389/fchem.2022.1089959
Abstract: Activity-based probes (ABP) are molecules that bind covalently to the active form of an enzyme family, making them an attractive tool for target and biomarker identification and drug discovery. The present study describes the synthesis… read more here.
Keywords: like serine; based probes; activity based; trypsin like ... See more keywords
Novel Inhibitors and Activity-Based Probes Targeting Trypsin-Like Serine Proteases
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Chemistry"
DOI: 10.3389/fchem.2022.782608
Abstract: The trypsin-like proteases (TLPs) play widespread and diverse roles, in a host of physiological and pathological processes including clot dissolution, extracellular matrix remodelling, infection, angiogenesis, wound healing and tumour invasion/metastasis. Moreover, these enzymes are involved… read more here.
Keywords: novel inhibitors; inhibitors activity; activity; activity based ... See more keywords
A Preliminary Study on the Ability of the Trypsin-Like Peptidase Activity Assay Kit to Detect Periodontitis
Sign Up to like & getrecommendations! Published in 2020 at "Dentistry Journal"
DOI: 10.3390/dj8030098
Abstract: This study aimed to explore whether the Trypsin-Like Peptidase Activity Assay Kit (TLP-AA-Kit), which measures the activity of N-benzoyl-dl-arginine peptidase (trypsin-like peptidase), can be used as a reliable tool for periodontitis detection in population-based surveillance.… read more here.
Keywords: trypsin like; like peptidase; kit; tlp kit ... See more keywords
Changes in Biochemical Properties and Activity of Trypsin-like Protease (Litopenaeus vannamei) Treated by Atmospheric Cold Plasma (ACP)
Sign Up to like & getrecommendations! Published in 2022 at "Foods"
DOI: 10.3390/foods11091277
Abstract: The changes in the functional properties of trypsin from shrimps (Litopenaeus vannamei) after, Atmospheric Cold Plasma (ACP) treatments, have been evaluated in terms of enzyme inactivation, surface hydrophobicity, secondary structure, fluorescence intensity, and particle size… read more here.
Keywords: atmospheric cold; litopenaeus vannamei; like protease; activity ... See more keywords