How a Fragment Draws Attention to Selectivity Discriminating Features between the Related Proteases Trypsin and Thrombin.
Sign Up to like & getrecommendations! Published in 2021 at "Journal of medicinal chemistry"
DOI: 10.1021/acs.jmedchem.0c01809
Abstract: In the S1 pocket, the serine proteases thrombin and trypsin commonly feature Asp189 and a Ala190Ser and Glu192Gln exchange. Nevertheless, thrombin cleaves peptide chains solely after Arg, and trypsin after Lys and Arg. Thrombin exhibits… read more here.
Keywords: draws attention; fragment draws; trypsin thrombin; selectivity ... See more keywords
Protein-induced Change in Ligand Protonation during Trypsin and Thrombin Binding: Hint on Differences in Selectivity Determinants of both Proteins?
Sign Up to like & getrecommendations! Published in 2020 at "Journal of medicinal chemistry"
DOI: 10.1021/acs.jmedchem.9b02061
Abstract: Trypsin and thrombin, structurally similar serine proteases, recognize different substrates; thrombin cleaves after Arg whereas trypsin after Lys/Arg. Both recognize basic substrate headgroups via Asp189 at the bottom of the S1-pocket. By crystallography and ITC,… read more here.
Keywords: trypsin thrombin; protein induced; trypsin; thrombin ... See more keywords