Single tube gene synthesis by phosphoramidate chemical ligation.
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DOI: 10.1039/c7cc00858a
Abstract: Templated chemical ligation of 5'-amino and 3'-phosphate oligonucleotides was used to synthesise a 762 base pair gene for green fluorescent protein. The phosphoramidate linkage can be read by DNA polymerase and transcribed to make RNA.… read more here.
Keywords: tube gene; chemical ligation; ligation; single tube ... See more keywords