Evaluating a New High-throughput Twin-Arginine Translocase Assay in Bacteria for Therapeutic Applications
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DOI: 10.1007/s00284-017-1321-z
Abstract: The twin-arginine translocase (Tat) pathway is involved in the transport of folded proteins in bacteria, and has been implicated in virulence and pathogenesis. A simple but efficient assay based on the quantification of the exopolysaccharide… read more here.
Keywords: arginine translocase; twin arginine; therapeutic applications; high throughput ... See more keywords
Twin-arginine signal peptide of Bacillus licheniformis GlmU efficiently mediated secretory expression of protein glutaminase
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DOI: 10.1016/j.ejbt.2019.10.006
Abstract: Abstract Background Protein glutaminase specifically deamidates glutamine residue in protein and therefore significantly improves protein solubility and colloidal stability of protein solution. In order to improve its preparation efficiency, we exploited the possibility for its… read more here.
Keywords: protein; twin arginine; glmu; expression ... See more keywords
Targeting of proteins to the twin‐arginine translocation pathway
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DOI: 10.1111/mmi.14461
Abstract: The twin‐arginine protein transport (Tat pathway) is found in prokaryotes and plant organelles and transports folded proteins across membranes. Targeting of substrates to the Tat system is mediated by the presence of an N‐terminal signal… read more here.
Keywords: twin arginine; translocation pathway; arginine translocation; proteins twin ... See more keywords
Signal Peptide Hydrophobicity Modulates Interaction with the Twin-Arginine Translocase
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DOI: 10.1128/mbio.00909-17
Abstract: ABSTRACT The general secretory pathway (Sec) and twin-arginine translocase (Tat) operate in parallel to export proteins across the cytoplasmic membrane of prokaryotes and the thylakoid membrane of plant chloroplasts. Substrates are targeted to their respective… read more here.
Keywords: signal peptides; signal peptide; tat pathway; twin arginine ... See more keywords
Cloning and Expression of Twin-Arginine Translocation D Family Deoxyribonuclease of Clostridium Chauvoei
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DOI: 10.18805/ijar.b-3855
Abstract: Clostridium chauvoei, an anaerobic bacterium reported worldwide, is responsible for Black Quarter, a dreadful disease of ruminants. This bacterium produces many toxins responsible for the pathogenesis of the disease. Except for the well-studied virulence factors… read more here.
Keywords: twin arginine; clostridium chauvoei; arginine translocation; expression ... See more keywords
An Engineered Outer Membrane-Defective Escherichia coli Secreting Protective Antigens against Streptococcus suis via the Twin-Arginine Translocation Pathway as a Vaccine
Sign Up to like & getrecommendations! Published in 2022 at "Journal of Microbiology and Biotechnology"
DOI: 10.4014/jmb.2107.07052
Abstract: Live bacterial vector vaccines are one of the most promising vaccine types and have the advantages of low cost, flexibility, and good safety. Meanwhile, protein secretion systems have been reported as useful tools to facilitate… read more here.
Keywords: outer membrane; antigen proteins; escherichia coli; arginine translocation ... See more keywords