In vitro profiling of toxicity and endocrine disrupting effects of bisphenol analogues by employing MCF‐7 cells and two‐hybrid yeast bioassay
Sign Up to like & getrecommendations! Published in 2017 at "Environmental Toxicology"
DOI: 10.1002/tox.22234
Abstract: The potentially adverse health implications of bisphenol A (BPA) have led to increasing use of alternative bisphenols (BPs). However, little is known about the toxicity of alternative BPs. In this study, the cytotoxicity, genotoxicity, intracellular… read more here.
Keywords: disrupting effects; mcf cells; endocrine disrupting; hybrid yeast ... See more keywords
Identification of PDZ Interactions by Yeast Two-Hybrid Technique.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1166-1_1
Abstract: The yeast two-hybrid technique is a powerful method to detect direct protein-protein interactions. Due to its accessibility, speed, and versatility, this technique is easy to set up in any laboratory and suitable for small and… read more here.
Keywords: technique; two hybrid; identification pdz; hybrid technique ... See more keywords
A Bacterial Adenylate Cyclase-Based Two-Hybrid System Compatible with Gateway® Cloning.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7871-7_6
Abstract: The bacterial adenylate cyclase two-hybrid system (BACTH) is a genetic approach used to test protein interactions in vivo in E. coli. This system takes advantage of the two catalytic domains of Bordetella pertussis adenylate cyclase… read more here.
Keywords: hybrid system; system; two hybrid; adenylate cyclase ... See more keywords
Assessing the feasibility of two hybrid MBR systems using PAC for removing macro and micropollutants.
Sign Up to like & getrecommendations! Published in 2017 at "Journal of environmental management"
DOI: 10.1016/j.jenvman.2016.03.023
Abstract: The removal of 10 organic micropollutants (OMPs) was studied in two MBRs using different types of membrane (flat sheet microfiltration, MF, and hollow fiber ultrafiltration, UF) operated under aerobic conditions with direct dosing of powdered… read more here.
Keywords: systems using; mbr systems; two hybrid; feasibility two ... See more keywords
A Modified Yeast Two-Hybrid Platform Enables Dynamic Control of Expression Intensities to Unmask Properties of Protein–Protein Interactions
Sign Up to like & getrecommendations! Published in 2022 at "ACS Synthetic Biology"
DOI: 10.1021/acssynbio.2c00192
Abstract: The yeast two-hybrid (Y2H) assay is widely used for protein–protein interaction characterization due to its simplicity and accessibility. However, it may mask changes in affinity caused by mutations or ligand activation due to signal saturation.… read more here.
Keywords: protein protein; two hybrid; expression; protein interactions ... See more keywords
Directed Evolution of Herbicide Biosensors in a Fluorescence-Activated Cell-Sorting-Compatible Yeast Two-Hybrid Platform
Sign Up to like & getrecommendations! Published in 2022 at "ACS Synthetic Biology"
DOI: 10.1021/acssynbio.2c00297
Abstract: Developing sensory modules for specific molecules of interest represents a fundamental challenge in synthetic biology and its applications. A somewhat generalizable approach for this challenge is demonstrated here by evolving a naturally occurring chemically induced… read more here.
Keywords: two hybrid; hybrid platform; directed evolution; yeast two ... See more keywords
A novel reverse two-hybrid method for the identification of missense mutations that disrupt protein–protein binding
Sign Up to like & getrecommendations! Published in 2020 at "Scientific Reports"
DOI: 10.1038/s41598-020-77992-1
Abstract: The reverse two-hybrid system is a powerful method to select mutations that disrupt the interaction between two proteins and therefore to identify the residues involved in this interaction. However, the usefulness of this technique has… read more here.
Keywords: protein; mutations disrupt; two hybrid; missense mutations ... See more keywords
A yeast two-hybrid screening identifies novel Atg8a interactors in Drosophila
Sign Up to like & getrecommendations! Published in 2022 at "Autophagy"
DOI: 10.1080/15548627.2022.2045535
Abstract: ABSTRACT Macroautophagy/autophagy-related protein Atg8/LC3 is important for autophagosome biogenesis and required for selective degradation of various substrates. In our recent study, we performed a yeast two-hybrid screening to identify proteins that interact with Atg8a, the… read more here.
Keywords: hybrid screening; two hybrid; novel atg8a; screening identifies ... See more keywords
Development and application of a recombination-based library versus library high- throughput yeast two-hybrid (RLL-Y2H) screening system
Sign Up to like & getrecommendations! Published in 2018 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkx1173
Abstract: Abstract Protein-protein interaction (PPI) network maintains proper function of all organisms. Simple high-throughput technologies are desperately needed to delineate the landscape of PPI networks. While recent state-of-the-art yeast two-hybrid (Y2H) systems improved screening efficiency, either… read more here.
Keywords: system; based library; two hybrid; recombination based ... See more keywords
Complete circularized genome data of two Spanish strains of Xylella fastidiosa (IVIA5235 and IVIA5901) using two hybrid assembly approaches.
Sign Up to like & getrecommendations! Published in 2020 at "Phytopathology"
DOI: 10.1094/phyto-01-20-0012-a
Abstract: Xylella fastidiosa is an economically important plant pathogenic bacterium of global importance associated, since 2013, with a devastating epidemic in olive trees in Italy. Since then, several outbreaks of this pathogen have been reported in… read more here.
Keywords: fastidiosa ivia5235; two spanish; spanish strains; xylella fastidiosa ... See more keywords
Novel binding partners for Prenylated Rab Acceptor 1 identified by a split-ubiquitin yeast two-hybrid screen
Sign Up to like & getrecommendations! Published in 2019 at "BMC Research Notes"
DOI: 10.1186/s13104-019-4219-y
Abstract: ObjectivePrenylated Rab Acceptor 1 (PRA1) is a transmembrane protein localized to the early secretory pathway. It has been found to interact with an array of Rab GTPases, leading to its hypothesized function in the recycling… read more here.
Keywords: split ubiquitin; binding partners; two hybrid; screen ... See more keywords