Genome editing in PDS genes of tomatoes by non-selection method and of Nicotiana benthamiana by one single guide RNA to edit two orthologs.
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DOI: 10.5511/plantbiotechnology.20.0527b
Abstract: The CRISPR/Cas9 system is widely used for targeted mutagenesis in many organisms including plants. For application of this system, tissue culture methods need to be established. In this study, detailed methods for introduction of mutations… read more here.
Keywords: two orthologs; one single; single guide; pds genes ... See more keywords