Heterologous gene expression and characterization of TK2246, a highly active and thermostable plant type l-asparaginase from Thermococcus kodakarensis.
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DOI: 10.1016/j.ijbiomac.2020.01.012
Abstract: The genome sequence of the hyperthermophilic archaeon Thermococcus kodakarensis contains two putative genes, TK1656 and TK2246, annotated as l-asparaginases. TK1656 has been reported previously. The current report is focused on TK2246, a plant-type l-asparaginase, which… read more here.
Keywords: plant type; gene; type asparaginase;
Structural Aspects of E. coli Type II Asparaginase in Complex with Its Secondary Product L-Glutamate
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DOI: 10.3390/ijms23115942
Abstract: Bacterial L-asparaginases are amidohydrolases (EC 3.5.1.1) capable of deaminating L-asparagine and, with reduced efficiency, L-glutamine. Interest in the study of L-asparaginases is driven by their use as biodrugs for the treatment of acute lymphoblastic leukemia.… read more here.
Keywords: secondary product; complex secondary; type asparaginase; asparaginase ... See more keywords
Cis-Element Engineering Promotes the Expression of Bacillus subtilis Type I L-Asparaginase and Its Application in Food
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DOI: 10.3390/ijms23126588
Abstract: Type I L-asparaginase from Bacillus licheniformis Z-1 (BlAase) was efficiently produced and secreted in Bacillus subtilis RIK 1285, but its low yield made it unsuitable for industrial use. Thus, a combined method was used in… read more here.
Keywords: promoter; type asparaginase; bacillus subtilis; blaase ... See more keywords