WT-PE: Prime editing with nuclease wild-type Cas9 enables versatile large-scale genome editing
Sign Up to like & getrecommendations! Published in 2022 at "Signal Transduction and Targeted Therapy"
DOI: 10.1038/s41392-022-00936-w
Abstract: Large scale genomic aberrations including duplication, deletion, translocation, and other structural changes are the cause of a subtype of hereditary genetic disorders and contribute to onset or progress of cancer. The current prime editor, PE2,… read more here.
Keywords: large scale; prime editing; type cas9; nuclease wild ... See more keywords
Structural insights into Type II-D Cas9 and its robust cleavage activity
Sign Up to like & getrecommendations! Published in 2025 at "Nature Communications"
DOI: 10.1038/s41467-025-62128-8
Abstract: Type II-D Cas9 proteins (Cas9d) are more compact than typical Type II-A/B/C Cas9s. Here, we demonstrate that NsCas9d from Nitrospirae bacterium RBG_13_39_12 derived from a metagenomic assembly exhibits robust dsDNA cleavage activity comparable to SpCas9… read more here.
Keywords: type cas9; cas9; cleavage activity; cleavage ... See more keywords