LAUSR

About LAUSR
Donate
Contact Us

Follow us:

You are not signed in:

Sign Up!


   Articles with "type crispr" as a keyword



Photo from wikipedia

Introducing a Spectrum of Long-Range Genomic Deletions in Human Embryonic Stem Cells Using Type I CRISPR-Cas.

Sign Up to like & get
recommendations! Published in 2019 at "Molecular cell"

DOI: 10.1016/j.molcel.2019.03.014

Abstract: CRISPR-Cas systems enable microbial adaptive immunity and provide eukaryotic genome editing tools. These tools employ a single effector enzyme of type II or V CRISPR to generate RNA-guided, precise genome breaks. Here we demonstrate the feasibility… read more here.

Keywords: crispr cas; type crispr; long range; crispr ... See more keywords
Photo from wikipedia

Structure of the DNA-Bound Spacer Capture Complex of a Type II CRISPR-Cas System

Sign Up to like & get
recommendations! Published in 2019 at "Molecular Cell"

DOI: 10.1016/j.molcel.2019.04.020

Abstract: Summary CRISPR and associated Cas proteins function as an adaptive immune system in prokaryotes to combat bacteriophage infection. During the immunization step, new spacers are acquired by the CRISPR machinery, but the molecular mechanism of… read more here.

Keywords: system; crispr; spacer capture; dna ... See more keywords

Uncovering the Distinct Properties of a Bacterial Type I-E CRISPR Activation System.

Sign Up to like & get
recommendations! Published in 2022 at "ACS synthetic biology"

DOI: 10.1021/acssynbio.1c00496

Abstract: Synthetic gene regulators based upon CRISPR-Cas systems offer programmable technologies to control gene expression in bacteria. Bacterial CRISPR activators (CRISPRa) have been developed that use engineered type II CRISPR-dCas9 to localize transcription activation domains near… read more here.

Keywords: system; crispr cas; activation; type crispr ... See more keywords

Harnessing the Endogenous Type I-F CRISPR/Cas System for Efficient Genome Engineering and Gene Repression in Pseudomonas chlororaphis LX24.

Sign Up to like & get
recommendations! Published in 2025 at "ACS synthetic biology"

DOI: 10.1021/acssynbio.5c00371

Abstract: Pseudomonas chlororaphis, a nonpathogenic plant growth-promoting rhizobacterium, holds immense potential for agricultural and industrial applications due to its ability to biosynthesize bioactive metabolites. However, the lack of efficient genetic tools has hindered its metabolic engineering.… read more here.

Keywords: chlororaphis lx24; system; type crispr; chlororaphis ... See more keywords

Structure and genome editing of type I-B CRISPR-Cas

Sign Up to like & get
recommendations! Published in 2024 at "Nature Communications"

DOI: 10.1038/s41467-024-48598-2

Abstract: Type I CRISPR-Cas systems employ multi-subunit effector Cascade and helicase-nuclease Cas3 to target and degrade foreign nucleic acids, representing the most abundant RNA-guided adaptive immune systems in prokaryotes. Their ability to cause long fragment deletions… read more here.

Keywords: crispr cas; type; type cascade; type crispr ... See more keywords

Engineered minimal type I CRISPR-Cas system for transcriptional activation and base editing in human cells

Sign Up to like & get
recommendations! Published in 2024 at "Nature Communications"

DOI: 10.1038/s41467-024-51695-x

Abstract: Type I CRISPR-Cas systems are widespread and have exhibited remarkable versatility and efficiency in genome editing and gene regulation in prokaryotes. However, due to the multi-subunit composition and large size, their application in eukaryotes has… read more here.

Keywords: crispr cas; type; human cells; base ... See more keywords

Repurposing endogenous type I-E CRISPR-Cas systems for natural product discovery in Streptomyces

Sign Up to like & get
recommendations! Published in 2024 at "Nature Communications"

DOI: 10.1038/s41467-024-54196-z

Abstract: The multifunctional proteins of class 2 CRISPR systems such as Cas9, have been employed to activate cryptic biosynthetic gene clusters (BGCs) in Streptomyces, which represent a large and hidden reservoir of natural products. However, such… read more here.

Keywords: endogenous type; natural product; type crispr; crispr ... See more keywords

Repurposing Type I-A CRISPR-Cas3 for a robust diagnosis of human papillomavirus (HPV)

Sign Up to like & get
recommendations! Published in 2024 at "Communications Biology"

DOI: 10.1038/s42003-024-06537-3

Abstract: R-loop-triggered collateral single-stranded DNA (ssDNA) nuclease activity within Class 1 Type I CRISPR-Cas systems holds immense potential for nucleic acid detection. However, the hyperactive ssDNase activity of Cas3 introduces unwanted noise and false-positive results. In… read more here.

Keywords: cas3; hpv; crispr cas3; type crispr ... See more keywords

Characterization and applications of Type I CRISPR-Cas systems.

Sign Up to like & get
recommendations! Published in 2020 at "Biochemical Society transactions"

DOI: 10.1042/bst20190119

Abstract: CRISPR-Cas constitutes the adaptive immune system of bacteria and archaea. This RNA-mediated sequence-specific recognition and targeting machinery has been used broadly for diverse applications in a wide range of organisms across the tree of life.… read more here.

Keywords: cas systems; cas; characterization applications; type crispr ... See more keywords

CRISPRtracrRNA: robust approach for CRISPR tracrRNA detection

Sign Up to like & get
recommendations! Published in 2022 at "Bioinformatics"

DOI: 10.1093/bioinformatics/btac466

Abstract: Abstract Motivation The CRISPR-Cas9 system is a Type II CRISPR system that has rapidly become the most versatile and widespread tool for genome engineering. It consists of two components, the Cas9 effector protein, and a… read more here.

Keywords: crispr; crisprtracrrna; rna; type crispr ... See more keywords

Discovery and characterization of novel type I-D CRISPR-guided transposons identified among diverse Tn7-like elements in cyanobacteria

Sign Up to like & get
recommendations! Published in 2022 at "Nucleic Acids Research"

DOI: 10.1093/nar/gkac1216

Abstract: Abstract CRISPR-Cas defense systems have been naturally coopted for guide RNA-directed transposition by Tn7 family bacterial transposons. We find cyanobacterial genomes are rich in Tn7-like elements, including most of the known guide RNA-directed transposons, the… read more here.

Keywords: crispr; like elements; type crispr; crispr cas ... See more keywords