Introducing a Spectrum of Long-Range Genomic Deletions in Human Embryonic Stem Cells Using Type I CRISPR-Cas.
Sign Up to like & getrecommendations! Published in 2019 at "Molecular cell"
DOI: 10.1016/j.molcel.2019.03.014
Abstract: CRISPR-Cas systems enable microbial adaptive immunity and provide eukaryotic genome editing tools. These tools employ a single effector enzyme of type II or V CRISPR to generate RNA-guided, precise genome breaks. Here we demonstrate the feasibility… read more here.
Keywords: crispr cas; type crispr; long range; crispr ... See more keywords
Structure of the DNA-Bound Spacer Capture Complex of a Type II CRISPR-Cas System
Sign Up to like & getrecommendations! Published in 2019 at "Molecular Cell"
DOI: 10.1016/j.molcel.2019.04.020
Abstract: Summary CRISPR and associated Cas proteins function as an adaptive immune system in prokaryotes to combat bacteriophage infection. During the immunization step, new spacers are acquired by the CRISPR machinery, but the molecular mechanism of… read more here.
Keywords: system; crispr; spacer capture; dna ... See more keywords
Uncovering the Distinct Properties of a Bacterial Type I-E CRISPR Activation System.
Sign Up to like & getrecommendations! Published in 2022 at "ACS synthetic biology"
DOI: 10.1021/acssynbio.1c00496
Abstract: Synthetic gene regulators based upon CRISPR-Cas systems offer programmable technologies to control gene expression in bacteria. Bacterial CRISPR activators (CRISPRa) have been developed that use engineered type II CRISPR-dCas9 to localize transcription activation domains near… read more here.
Keywords: system; crispr cas; activation; type crispr ... See more keywords
Characterization and applications of Type I CRISPR-Cas systems.
Sign Up to like & getrecommendations! Published in 2020 at "Biochemical Society transactions"
DOI: 10.1042/bst20190119
Abstract: CRISPR-Cas constitutes the adaptive immune system of bacteria and archaea. This RNA-mediated sequence-specific recognition and targeting machinery has been used broadly for diverse applications in a wide range of organisms across the tree of life.… read more here.
Keywords: cas systems; cas; characterization applications; type crispr ... See more keywords
CRISPRtracrRNA: robust approach for CRISPR tracrRNA detection
Sign Up to like & getrecommendations! Published in 2022 at "Bioinformatics"
DOI: 10.1093/bioinformatics/btac466
Abstract: Abstract Motivation The CRISPR-Cas9 system is a Type II CRISPR system that has rapidly become the most versatile and widespread tool for genome engineering. It consists of two components, the Cas9 effector protein, and a… read more here.
Keywords: crispr; crisprtracrrna; rna; type crispr ... See more keywords
Discovery and characterization of novel type I-D CRISPR-guided transposons identified among diverse Tn7-like elements in cyanobacteria
Sign Up to like & getrecommendations! Published in 2022 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkac1216
Abstract: Abstract CRISPR-Cas defense systems have been naturally coopted for guide RNA-directed transposition by Tn7 family bacterial transposons. We find cyanobacterial genomes are rich in Tn7-like elements, including most of the known guide RNA-directed transposons, the… read more here.
Keywords: crispr; like elements; type crispr; crispr cas ... See more keywords
Functional characterization of diverse type I-F CRISPR-associated transposons
Sign Up to like & getrecommendations! Published in 2022 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkac985
Abstract: Abstract CRISPR-Cas systems generally provide adaptive immunity in prokaryotes through RNA-guided degradation of foreign genetic elements like bacteriophages and plasmids. Recently, however, transposon-encoded and nuclease-deficient CRISPR-Cas systems were characterized and shown to be co-opted by… read more here.
Keywords: crispr; type crispr; rna guided; crispr cas ... See more keywords
Type IV CRISPR–Cas systems are highly diverse and involved in competition between plasmids
Sign Up to like & getrecommendations! Published in 2019 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkz1197
Abstract: Abstract CRISPR–Cas systems provide prokaryotes with adaptive immune functions against viruses and other genetic parasites. In contrast to all other types of CRISPR–Cas systems, type IV has remained largely overlooked. Here, we describe a previously… read more here.
Keywords: type; crispr cas; cas systems; type crispr ... See more keywords
Structural insights into the modulatory role of the accessory protein WYL1 in the Type VI-D CRISPR-Cas system
Sign Up to like & getrecommendations! Published in 2019 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkz269
Abstract: Abstract The Type VI-D CRISPR-Cas system employs an RNA-guided RNase Cas13d with minimal targeting constraints to combat viral infections. This CRISPR system contains RspWYL1 as a unique accessory protein that plays a key role in… read more here.
Keywords: system; crispr cas; cas system; type crispr ... See more keywords
Target degradation of Type I CRISPR
Sign Up to like & getrecommendations! Published in 2018 at "Science"
DOI: 10.1126/science.361.6397.38-i
Abstract: Structural Biology The CRISPR adaptive immune systems defend bacteria against invaders. Type I CRISPR-Cas systems, the most prevalent type, use a Cascade complex to search the target DNA that is then degraded by Cas3 protein.… read more here.
Keywords: type; target degradation; type crispr; dna ... See more keywords
Functionally diverse type V CRISPR-Cas systems
Sign Up to like & getrecommendations! Published in 2019 at "Science"
DOI: 10.1126/science.aav7271
Abstract: Additional, diverse CRISPR systems CRISPR systems have been revolutionizing molecular biology. Mining the metagenomic database, Yan et al. systematically discovered additional subtypes of type V CRISPR-Cas systems. The additional Cas12 effectors displayed a range of… read more here.
Keywords: crispr; crispr cas; cas12; cas systems ... See more keywords