Assembly of Complex Pathways Using Type IIs Restriction Enzymes.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9142-6_7
Abstract: Efficient DNA assembly methods are essential tools for synthetic biology and metabolic engineering. Among several recently developed methods that allow assembly of multiple DNA fragments in a single step, DNA assembly using type IIS enzymes… read more here.
Keywords: multigene constructs; assembly complex; constructs using; using type ... See more keywords
Type IIs restriction based combinatory modulation technique for metabolic pathway optimization
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DOI: 10.1186/s12934-017-0659-z
Abstract: BackgroundOne of the most important research subjects of metabolic engineering is pursuing a balanced metabolic pathway, which is the basis of an efficient cell factory. In this work, we dedicated to develop a simple and… read more here.
Keywords: technique; metabolic pathway; iis restriction; pathway ... See more keywords
Having a Same Type IIS Enzyme’s Restriction Site on Guide RNA Sequence Does Not Affect Golden Gate (GG) Cloning and Subsequent CRISPR/Cas Mutagenesis
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DOI: 10.3390/ijms23094889
Abstract: Golden gate/modular cloning facilitates faster and more efficient cloning by utilizing the unique features of the type IIS restriction enzymes. However, it is known that targeted insertion of DNA fragment(s) must not include internal type… read more here.
Keywords: golden gate; type iis; restriction; crispr ... See more keywords