An enterococcal phage protein inhibits type IV restriction enzymes involved in antiphage defense
Sign Up to like & getrecommendations! Published in 2024 at "Nature Communications"
DOI: 10.1038/s41467-024-51346-1
Abstract: The prevalence of multidrug resistant (MDR) bacterial infections continues to rise as the development of antibiotics needed to combat these infections remains stagnant. MDR enterococci are a major contributor to this crisis. A potential therapeutic… read more here.
Keywords: phage; antiphage defense; restriction; type restriction ... See more keywords
Modified DNA substrate selectivity by GmrSD-family Type IV restriction enzyme BrxU
Sign Up to like & getrecommendations! Published in 2025 at "Philosophical Transactions of the Royal Society B: Biological Sciences"
DOI: 10.1098/rstb.2024.0072
Abstract: Bacteriophages (phages), viral predators of bacteria, generate selection pressure that causes bacteria to evolve defence systems. Type I, II and III restriction enzymes cleave incoming non-modified phage DNAs. Phages have evolved to defend against these… read more here.
Keywords: modified dna; restriction; type restriction; gmrsd family ... See more keywords
Natural Recombination among Type I Restriction-Modification Systems Creates Diverse Genomic Methylation Patterns among Xylella fastidiosa Strains
Sign Up to like & getrecommendations! Published in 2022 at "Applied and Environmental Microbiology"
DOI: 10.1128/aem.01873-22
Abstract: Economic impacts on agricultural production due to X. fastidiosa have been severe in the Americas, Europe, and parts of Asia. Despite a long history of research on this pathogen, certain fundamental questions regarding the biology,… read more here.
Keywords: methylation; recombination; fastidiosa; fastidiosa strains ... See more keywords
Vibrio cholerae pathogenicity island 2 encodes two distinct types of restriction systems
Sign Up to like & getrecommendations! Published in 2024 at "Journal of Bacteriology"
DOI: 10.1128/jb.00145-24
Abstract: ABSTRACT In response to predation by bacteriophages and invasion by other mobile genetic elements such as plasmids, bacteria have evolved specialized defense systems that are often clustered together on genomic islands. The O1 El Tor… read more here.
Keywords: system; pathogenicity island; restriction; type restriction ... See more keywords
Deletion of the Zinc Transporter Lipoprotein AdcAII Causes Hyperencapsulation of Streptococcus pneumoniae Associated with Distinct Alleles of the Type I Restriction-Modification System
Sign Up to like & getrecommendations! Published in 2020 at "mBio"
DOI: 10.1128/mbio.00445-20
Abstract: The Streptococcus pneumoniae capsule affects multiple interactions with the host including contributing to colonization and immune evasion. During infection, the capsule thickness varies, but the mechanisms regulating this are poorly understood. We have identified an… read more here.
Keywords: type restriction; system; capsule thickness; capsule ... See more keywords
Golden Gate assembly of BioBrick-compliant parts using Type II restriction endonucleases.
Sign Up to like & getrecommendations! Published in 2022 at "BioTechniques"
DOI: 10.2144/btn-2021-0083
Abstract: Aims: New methods of DNA recombination that capture the principal advantages of the BioBrick standard (ease of design) and Golden Gate assembly (decreased labor) are demonstrated here. Methods & materials: Both methods employ DNA methyltransferase… read more here.
Keywords: golden gate; restriction endonucleases; gate assembly; assembly ... See more keywords