Natural Recombination among Type I Restriction-Modification Systems Creates Diverse Genomic Methylation Patterns among Xylella fastidiosa Strains
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DOI: 10.1128/aem.01873-22
Abstract: Economic impacts on agricultural production due to X. fastidiosa have been severe in the Americas, Europe, and parts of Asia. Despite a long history of research on this pathogen, certain fundamental questions regarding the biology,… read more here.
Keywords: methylation; recombination; fastidiosa; fastidiosa strains ... See more keywords
Deletion of the Zinc Transporter Lipoprotein AdcAII Causes Hyperencapsulation of Streptococcus pneumoniae Associated with Distinct Alleles of the Type I Restriction-Modification System
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DOI: 10.1128/mbio.00445-20
Abstract: The Streptococcus pneumoniae capsule affects multiple interactions with the host including contributing to colonization and immune evasion. During infection, the capsule thickness varies, but the mechanisms regulating this are poorly understood. We have identified an… read more here.
Keywords: type restriction; system; capsule thickness; capsule ... See more keywords
Golden Gate assembly of BioBrick-compliant parts using Type II restriction endonucleases.
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DOI: 10.2144/btn-2021-0083
Abstract: Aims: New methods of DNA recombination that capture the principal advantages of the BioBrick standard (ease of design) and Golden Gate assembly (decreased labor) are demonstrated here. Methods & materials: Both methods employ DNA methyltransferase… read more here.
Keywords: golden gate; restriction endonucleases; gate assembly; assembly ... See more keywords