M379A Mutant Tyrosine Phenol‐lyase from Citrobacter freundii Has Altered Conformational Dynamics
Sign Up to like & getrecommendations! Published in 2022 at "Chembiochem"
DOI: 10.1002/cbic.202200028
Abstract: The M379A mutant of Citrobacter freundii tyrosine phenol‐lyase (TPL) has been prepared. M379A TPL is a robust catalyst to prepare a number of tyrosines substituted at the 3‐position with bulky groups that cannot be made… read more here.
Keywords: tyrosine phenol; m379a mutant; m379a tpl; citrobacter freundii ... See more keywords
Efficient strategies to enhance plasmid stability for fermentation of recombinant Escherichia coli harboring tyrosine phenol lyase.
Sign Up to like & getrecommendations! Published in 2021 at "Biotechnology letters"
DOI: 10.1007/s10529-021-03082-5
Abstract: OBJECTIVE To solve the bottleneck of plasmid instability during microbial fermentation of L-DOPA with recombinant Escherichia coli expressing heterologous tyrosine phenol lyase. RESULTS The tyrosine phenol lyase from Fusobacterium nucleatum was constitutively expressed in E.… read more here.
Keywords: plasmid stability; phenol lyase; fermentation; tyrosine phenol ... See more keywords
Regioselective Biocatalytic Transformations Employing Transaminases and Tyrosine Phenol Lyases
Sign Up to like & getrecommendations! Published in 2018 at "Topics in Catalysis"
DOI: 10.1007/s11244-018-1054-7
Abstract: Regioselective reactions allow the differentiation between two or more chemically identical reactive centers within the same molecule. They are highly desirable transformations in organic synthesis, as they avoid additional chemical operations and sophisticated protection/deprotection strategies.… read more here.
Keywords: phenol lyases; employing transaminases; regioselective biocatalytic; tyrosine phenol ... See more keywords
High-throughput assay of tyrosine phenol-lyase activity using a cascade of enzymatic reactions.
Sign Up to like & getrecommendations! Published in 2022 at "Analytical biochemistry"
DOI: 10.1016/j.ab.2022.114547
Abstract: Tyrosine phenol-lyase (TPL) exhibits great potential in industrial biosynthesis of l-tyrosine and its derivates. To uncover and screen TPLs with excellent catalytic properties, there is unmet demand for development of facile and reliable screening system… read more here.
Keywords: phenol lyase; catechol; tyrosine phenol; activity ... See more keywords
Statistical medium optimization and DO-STAT fed-batch fermentation for enhanced production of tyrosine phenol lyase in recombinant Escherichia coli
Sign Up to like & getrecommendations! Published in 2019 at "Preparative Biochemistry and Biotechnology"
DOI: 10.1080/10826068.2018.1541808
Abstract: Abstract Tyrosine phenol lyase (TPL) is a robust biocatalyst for the production of L-dihydroxyphenylalanine (L-DOPA). The improvement of TPL production is conducive to the industrial potential. In this study, the optimization of culture medium of… read more here.
Keywords: recombinant escherichia; production; escherichia coli; tyrosine phenol ... See more keywords