An optical and non‐invasive method to detect the accumulation of ubiquitin chains
Sign Up to like & getrecommendations! Published in 2019 at "Cell Biology International"
DOI: 10.1002/cbin.11186
Abstract: The accumulations of excess amounts of polyubiquitinated proteins are cytotoxic and frequently observed in pathologic tissue from patients of neurodegenerative diseases. Therefore, optical and non‐invasive methods to detect the increase of the amounts of polyubiquitinated… read more here.
Keywords: invasive method; polyubiquitinated proteins; optical non; ubiquitin ... See more keywords
Proteasome-mediated protein degradation is enhanced by fusion ubiquitin with unstructured degron.
Sign Up to like & getrecommendations! Published in 2018 at "Biochemical and biophysical research communications"
DOI: 10.1016/j.bbrc.2018.05.088
Abstract: Methods to induce proteasomal degradation of unwanted proteins are valuable in biomedical studies and thus receive increasing attention. For efficient degradation, the proteasome requires both a ubiquitin tag, which delivers substrates to the proteasome, and… read more here.
Keywords: degradation; proteasome mediated; degron; ubiquitin ... See more keywords
Crystal structure of the Ube2K/E2-25K and K48-linked di-ubiquitin complex provides structural insight into the mechanism of K48-specific ubiquitin chain synthesis.
Sign Up to like & getrecommendations! Published in 2018 at "Biochemical and biophysical research communications"
DOI: 10.1016/j.bbrc.2018.10.067
Abstract: Ubiquitin-conjugating enzymes (E2) form thioester bonds with ubiquitin (Ub), which are subsequently transferred to target proteins for cellular progress. Ube2K/E2-25K (a class II E2 enzyme) contains a C-terminal ubiquitin-associated (UBA) domain that has been suggested… read more here.
Keywords: crystal structure; k48; ube2k 25k; k48 linked ... See more keywords
Branching Out: Improved Signaling by Heterotypic Ubiquitin Chains.
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DOI: 10.1016/j.tcb.2019.06.003
Abstract: Ubiquitin chains of distinct topologies control the stability, interactions, or localization of many proteins in eukaryotic cells, and thus play an essential role in cellular information transfer. It has recently been found that ubiquitin chains… read more here.
Keywords: signaling heterotypic; branching improved; ubiquitin; heterotypic ubiquitin ... See more keywords
Efficient semi-synthesis of ubiquitin-7-amino-4-methylcoumarin
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DOI: 10.1016/j.tet.2018.05.081
Abstract: Abstract Ubiquitin (Ub)-based probe Ub-7-amino-4-methylcoumarin is widely used to detect deubiquitinase (DUB) enzymatic activity, but its current synthesis procedures are cumbersome. In this work, we reported a new highly efficient semi-synthetic strategy to prepare Ub-AMC… read more here.
Keywords: semi synthesis; ubiquitin; efficient semi; amino methylcoumarin ... See more keywords
Conformational and functional characterization of artificially conjugated non-canonical ubiquitin dimers
Sign Up to like & getrecommendations! Published in 2019 at "Scientific Reports"
DOI: 10.1038/s41598-019-56458-z
Abstract: Ubiquitylation is an eminent posttranslational modification referring to the covalent attachment of single ubiquitin molecules or polyubiquitin chains to a target protein dictating the fate of such labeled polypeptide chains. Here, we have biochemically produced… read more here.
Keywords: artificially conjugated; functional characterization; ubiquitin dimers; ubiquitin ... See more keywords